|GEISER, DAVID - Pennsylvania State University|
|ALBERTS, HANNEKE - Cape Peninsula University Of Technology|
|RHEEDER, JOHN - Cape Peninsula University Of Technology|
Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 7/19/2017
Publication Date: N/A
Technical Abstract: Fusarium ranks one of the world’s most economically destructive and taxonomically challenging genera of mycotoxigenic plant pathogens. This is reflected, in part, by the numerous efforts to catalog its taxonomic diversity and mycotoxin potential over the past half century. Given the significant threat that fusarial toxins pose to agricultural biosecurity, food safety, and plant and animal health, several compendia have attempted to catalog the diversity of toxigenic fusaria and the toxins they produce. Marasas, Nelson & Toussoun’s 1984 compendium “Toxigenic Fusarium Species: Identity and Mycotoxicology” occupies a special place in the Fusarium mycotoxicology literature because the authors tested a number of the strains in this treatise for toxins, and because all of the strains are archived in the South African Medical Research Council (MRC) and Fusarium Research Center (FRC) Culture Collections. Given the transformative impact genealogical concordance phylogenetic species recognition (GCPSR)-based studies have had on Fusarium systematics over the past two decades, the present study was initiated to: (i) reassess species identity and phylogenetic diversity of 145 MRC strains via GCPSR, (ii) predict mycotoxin potential of any putatively novel Fusarium species discovered within the MRC collection by mining their whole genomes for biosynthetic pathways that encode mycotoxins, and (iii) test the MRC strains for mycotoxin production in liquid media and/or solid grain cultures using gas chromatography-mass spectrometry and high performance liquid chromatography-mass spectrometry.