Location: Natural Products Utilization ResearchTitle: Evaluation of secondary metabolites from the Red Sea tunicate polyclinum constellatum
|RASLAN, ALI - University Of Mississippi|
|RADWAN, MOHAMED - University Of Mississippi|
|AHMED, SAFWAT - Suez Canal University|
|NAFADY, ALAA - Al-Azhar University|
|WANAS, AMIRA - University Of Mississippi|
|BABU, TEKWANI - University Of Mississippi|
|HASSANIN, HASHIM - Suez Canal University|
|ELSOHLY, MAHMOUD - University Of Mississippi|
Submitted to: Pharmacy & Pharmacology International Journal
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 5/3/2017
Publication Date: 5/26/2017
Citation: Raslan, A., Radwan, M., Ahmed, S., Nafady, A., Wanas, A., Babu, T., Hassanin, H., Elsohly, M. 2017. Evaluation of secondary metabolites from the Red Sea tunicate polyclinum constellatum. Pharmacy & Pharmacology International Journal. 5(3): doi:10.15406/ppij.2017.05.00123
Interpretive Summary: Nine known compounds were isolated from the Red Sea tunicate Polyclinum constellatum. The structures were elucidated by spectroscopic analysis. The antimicrobial, antimalarial, antiprotzoal, and cytotoxic activities of the isolated compounds were evaluated. Compound 5 displayed good antitrypanocidal activity with IC50 and IC90 of 3.39 and 6.69 µg/mL respectively, and cytotoxicity against LLC-PK-1 with IC50 value of 23 µg/mL.
Technical Abstract: Chemical investigation of the Red Sea tunicate Polyclinum constellatum afforded nine compounds, identified as thymidine (1), uridine (2), adenosine (3), inosine (4), 24-methylene cholesterol (5), dihydrocholesterol (6), cholesterol (7), oleic acid (8) and 1,3-palmityl-2-palmitoleoylglycerol (9). All compounds isolated for the first time from the genus polyclinum. Acetylation of compounds 1, 2, 3, 4 and 5 yielded thymidine 3',5'-diacetate (1-Ac), uridine 2',3',5'-triacetate (2-Ac), adenosine 2',3',5'-triacetate (3-Ac), inosine 2',3',5'-triacetate (2-Ac) and 24-methylene cholesterol-3-acetate (5-Ac), respectively. Compound 5 showed potent antitrypanocidal activity with IC50 and IC90 values of 3.39 and 6.69 µg/mL, comparable to a-difluoromethylornithine (3.58 and 8.53 µg/mL). The overall findings of the present study have shown that the Red Sea tunicate Polyclinum constellatum revealed different varieties of secondary metabolites with antiprotozoal and cytotoxic activities.