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ARS Home » Southeast Area » Oxford, Mississippi » Natural Products Utilization Research » Research » Publications at this Location » Publication #342282

Research Project: Discovery and Development of Natural Products for Pharmaceutical and Agrochemical Applications II

Location: Natural Products Utilization Research

Title: Evaluation of secondary metabolites from the Red Sea tunicate polyclinum constellatum

item RASLAN, ALI - University Of Mississippi
item RADWAN, MOHAMED - University Of Mississippi
item AHMED, SAFWAT - Suez Canal University
item NAFADY, ALAA - Al-Azhar University
item WANAS, AMIRA - University Of Mississippi
item BABU, TEKWANI - University Of Mississippi
item HASSANIN, HASHIM - Suez Canal University
item ELSOHLY, MAHMOUD - University Of Mississippi

Submitted to: Pharmacy & Pharmacology International Journal
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 5/3/2017
Publication Date: 5/26/2017
Citation: Raslan, A., Radwan, M., Ahmed, S., Nafady, A., Wanas, A., Babu, T., Hassanin, H., Elsohly, M. 2017. Evaluation of secondary metabolites from the Red Sea tunicate polyclinum constellatum. Pharmacy & Pharmacology International Journal. 5(3): doi:10.15406/ppij.2017.05.00123

Interpretive Summary: Nine known compounds were isolated from the Red Sea tunicate Polyclinum constellatum. The structures were elucidated by spectroscopic analysis. The antimicrobial, antimalarial, antiprotzoal, and cytotoxic activities of the isolated compounds were evaluated. Compound 5 displayed good antitrypanocidal activity with IC50 and IC90 of 3.39 and 6.69 µg/mL respectively, and cytotoxicity against LLC-PK-1 with IC50 value of 23 µg/mL.

Technical Abstract: Chemical investigation of the Red Sea tunicate Polyclinum constellatum afforded nine compounds, identified as thymidine (1), uridine (2), adenosine (3), inosine (4), 24-methylene cholesterol (5), dihydrocholesterol (6), cholesterol (7), oleic acid (8) and 1,3-palmityl-2-palmitoleoylglycerol (9). All compounds isolated for the first time from the genus polyclinum. Acetylation of compounds 1, 2, 3, 4 and 5 yielded thymidine 3',5'-diacetate (1-Ac), uridine 2',3',5'-triacetate (2-Ac), adenosine 2',3',5'-triacetate (3-Ac), inosine 2',3',5'-triacetate (2-Ac) and 24-methylene cholesterol-3-acetate (5-Ac), respectively. Compound 5 showed potent antitrypanocidal activity with IC50 and IC90 values of 3.39 and 6.69 µg/mL, comparable to a-difluoromethylornithine (3.58 and 8.53 µg/mL). The overall findings of the present study have shown that the Red Sea tunicate Polyclinum constellatum revealed different varieties of secondary metabolites with antiprotozoal and cytotoxic activities.