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ARS Home » Pacific West Area » Davis, California » Crops Pathology and Genetics Research » Research » Publications at this Location » Publication #340779

Research Project: Sustainable Vineyard Production Systems

Location: Crops Pathology and Genetics Research

Title: Neofusicoccum parvum colonization of the grapevine woody stem triggers asynchronous host responses at the site of infection and in the leaves

Author
item Massonnet, Melanie - University Of California
item Galarneau, Erin
item Figueroa-balderas, Rosa - University Of California
item Lawrence, Daniel
item Miki, Shiho - Shimane University
item Sun, Qiang - University Of Wisconsin
item Wallis, Christopher
item Baumgartner, Kendra
item Cantu, Dario - University Of California

Submitted to: Frontiers in Plant Science
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 6/9/2017
Publication Date: 6/28/2017
Citation: Massonnet, M., Galarneau, E.R., Figueroa-Balderas, R., Lawrence, D.P., Miki, S., Sun, Q., Wallis, C.M., Baumgartner, K., Cantu, D. 2017. Neofusicoccum parvum colonization of the grapevine woody stem triggers asynchronous host responses at the site of infection and in the leaves. Frontiers in Plant Science. 8:1117. https://doi.org/10.3389/fpls.2017.01117.
DOI: https://doi.org/10.3389/fpls.2017.01117

Interpretive Summary: Grapevine trunk diseases cause important economic losses in vineyards worldwide. Neofusicoccum parvum, one of the most aggressive causal fungi of the trunk disease Botryosphaeria dieback, infects grapevines through pruning wounds. The fungi causes a chronic infection of the grapevine wood, leading to the formation of an internal canker. Symptoms of the disease then extend to shoots, with wilting of leaves and bud mortality. Our aim was to characterize the transcriptional dynamics of grapevine genes in the woody stem and in the leaves during N. parvum colonization. Genome-wide transcriptional profiling at seven distinct time points (0, 3, and 24 hours; 2, 6, and 8 weeks) showed that both stems and leaves undergo extensive transcriptomic reprogramming in response to infection of the stem. While most intense transcriptional responses were detected in the stems at 24 hours, responses were not detected in the leaves until the next sampling point at 2 weeks post-inoculation. Network co-expression analysis identified modules of co-expressed genes common to both organs and showed most of these genes were asynchronously modulated. The temporal shift between stem vs. leaf responses affected transcriptional modulation of genes involved in both signal perception and transduction, as well as downstream biological processes, including oxidative stress, cell wall rearrangement, and cell death. Promoter analysis of co-expressed genes identified common transcriptional regulators that the temporal shift may be due to asynchronous co-regulation. Topology analysis of stem and leaf co-expression networks pointed to specific transcription factor-encoding genes, which may be associated with the observed transcriptional responses in the two organs.

Technical Abstract: Grapevine trunk diseases cause important economic losses in vineyards worldwide. Neofusicoccum parvum, one of the most aggressive causal agents of the trunk disease Botryosphaeria dieback, colonizes cells and tissues of the grapevine wood, leading to the formation of an internal canker. Symptoms then extend to distal shoots, with wilting of leaves and bud mortality. Our aim was to characterize the transcriptional dynamics of grapevine genes in the woody stem and in the leaves during N. parvum colonization. Genome-wide transcriptional profiling at seven distinct time points (0, 3, and 24 hours; 2, 6, and 8 weeks) showed that both stems and leaves undergo extensive transcriptomic reprogramming in response to infection of the stem. While most intense transcriptional responses were detected in the stems at 24 hours, responses were not detected in the leaves until the next sampling point at 2 weeks post-inoculation. Network co-expression analysis identified modules of co-expressed genes common to both organs and showed most of these genes were asynchronously modulated. The temporal shift between stem vs. leaf responses affected transcriptional modulation of genes involved in both signal perception and transduction, as well as downstream biological processes, including oxidative stress, cell wall rearrangement, and cell death. Promoter analysis of co-expressed genes identified common transcriptional regulators that the temporal shift may be due to asynchronous co-regulation. Topology analysis of stem and leaf co-expression networks pointed to specific transcription factor-encoding genes, which may be associated with the observed transcriptional responses in the two organs.