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ARS Home » Midwest Area » Peoria, Illinois » National Center for Agricultural Utilization Research » Plant Polymer Research » Research » Publications at this Location » Publication #340111

Research Project: Conversion of Polysaccharides and Other Bio-based Materials to High-Value, Commercial Products

Location: Plant Polymer Research

Title: A rapid and specific biosensor for Salmonella typhimurium detection in milk

item ALESANDRE, D - Universidade Estadual Do Ceara
item MELO, A.M. - Universidade Estadual Do Ceara
item FURTADO, R - Embrapa
item BORGES, M - Universidade Estadual Do Ceara
item FIGUEIREDO, E.A. - Universidade Estadual Do Ceara
item Biswas, Atanu
item Cheng, Huai
item ALVES, C - Universidade Estadual Do Ceara

Submitted to: Food and Bioprocess Technology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 12/21/2017
Publication Date: 1/2/2018
Citation: Alesandre, D.L., Melo, A.A., Furtado, R.F., Borges, M.F., Figueiredo, E.T., Biswas, A., Cheng, H.N., Alves, C.R. 2018. A rapid and specific biosensor for Salmonella typhimurium detection in milk. Food and Bioprocess Technology. 11(4):748-756.

Interpretive Summary: Salmonella spp. is the major cause of food-borne diseases worldwide and its prevention is of high importance. This paper reports an amperometric biosensor for rapid and sensitive Salmonella Typhimurium detection in milk. An efficient amperometric biosensor for Salmonella Typhimurium detection was developed. The performance of this device is advantageous because it does not require a pre-enrichment step and takes a short time to obtain the results. This aspect is very important for food suppliers that need constant monitoring of food quality. The applicability of this method in milk samples was proven in skim and whole UHT milk. This research will help the food industry, especially those who can potentially be affected by Salmonella.

Technical Abstract: This paper reports the application of an amperometric biosensor for rapid and specific Salmonella Typhimurium detection in milk. This device was developed from self-assembled monolayer technique on a gold screen-printed electrode, using cysteamine thiol. Polyclonal antibodies were oriented by protein A immobilization. The biosensor structure was characterized by cyclic voltammetry, Fourier transform infrared spectroscopy, and scanning electron microscopy. The analytical response was obtained by a chronoamperometry technique, using a direct-sandwich peroxidase-labeled system. The biosensor device showed a qualitative behavior with a very low limit of detection of 10 CFU mL-1 and a detection time of 125 min. The biosensor specificity was demonstrated in pure and mixed samples with strains of Escherichia coli and Citrobacter freundii. The performance of the biosensor was found satisfactory, and the device was tested in skimmed and whole milk samples, being able to detect S. Typhimurium quickly, without an enrichment step. This structure of immunosensor assembly can be expended in future studies for other food matrices and bacterial species, making it a useful tool to ensure food safety.