|WANG, HONG-QI - Henan Agricultural University|
|WANG, JUN-YAN - Henan Agricultural University|
|HONG, HUI-JIE - Henan Agricultural University|
|YIN, HAI-YAN - Henan Agricultural University|
|ZHANG, LING - Henan Agricultural University|
|LIU, JI-HONG - Henan Agricultural University|
Submitted to: Quality and Safety of Agro-Products
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 3/15/2017
Publication Date: 3/17/2017
Publication URL: http://handle.nal.usda.gov/10113/5832874
Citation: Wang, H., Wang, J., Hong, H., Yin, H., Maragos, C.M., Zhang, L., Liu, J. 2017. Selection and application of strand displacement probes for a fumonisin B1 aptamer. Quality and Safety of Agro-Products. 1:44-48.
Interpretive Summary: Fumonisin B1 (FB1) is a mycotoxin commonly found in maize and maize products throughout the world. In this research we developed a novel method for detecting FB1 in maize. The method relies upon the binding of the toxin to an oligonucleotide (aptamer). A technique called fluorescence polarization was used to indirectly measure the presence of FB1. The technique is easy to operate and uses relatively low cost instrumentation and it may find application for the detection of this toxin in maize in place of the more traditional antibody-based screening assays.
Technical Abstract: Fumonisin B1 (FB1) is a toxin produced by Fusarium moniliforme, mainly on contaminated maize and maize products. In this study a solid surface chain displacement strategy was used to isolate oligonucleotide displacement probes for a FB1 aptamer. The probes were used as the basis for the development of a fluorescence polarization detection method for FB1 in corn. In actual samples the dynamic response range was 10 nM to 200 nM. The method is a homogeneous detection technology. It has low cost, good reproducibility, is easy to operate, and can be used to process a large number of samples for parallel detection.