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ARS Home » Plains Area » Fargo, North Dakota » Edward T. Schafer Agricultural Research Center » Cereal Crops Research » Research » Publications at this Location » Publication #338530

Research Project: HOST-PATHOGEN INTERACTIONS IN BARLEY AND WHEAT

Location: Cereal Crops Research

Title: Characterizing the Pyrenophora teres f. maculata – barley interaction using pathogen genetics

Author
item Carlsen, Steven - North Dakota State University
item Neupane, Anjan - North Dakota State University
item Wyatt, Nathan - North Dakota State University
item Richards, Jonathan - North Dakota State University
item Faris, Justin
item Xu, Steven
item Brueggeman, Robert - North Dakota State University
item Friesen, Timothy

Submitted to: Genes, Genomes, Genetics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 5/31/2017
Publication Date: 6/28/2017
Citation: Carlsen, S.A., Neupane, A., Wyatt, N.A., Richards, J.K., Faris, J.D., Xu, S.S., Brueggeman, R.S., Friesen, T.L. 2017. Characterizing the Pyrenophora teres f. maculata – barley interaction using pathogen genetics. G3: Genes, Genomes, Genetics. 7(8):2615-2626. 10.1534/g3.117.043265.
DOI: https://doi.org/10.1534/g3.117.043265

Interpretive Summary: Pyrenophora teres f. maculata causes spot form net blotch on barley. Current literature has only given us a glimpse into the complexity of this interaction with the report of several quantitative resistance/susceptibility genes on the host side as well as indications of several corresponding virulence/avirulence factors present in the global pathogen population based on the evaluations of pathogen populations on host differential sets. This study makes a major stride in understanding the genetics underlying the P. teres f. maculata-barley pathosystem by showing the complexity of virulence in this interaction, laying the groundwork for further characterization of the system.

Technical Abstract: Pyrenophora teres f. maculata is the cause of the foliar disease spot form net blotch (SFNB) on barley. To evaluate pathogen genetics underlying the P. teres f. maculata- barley interaction, we developed a 105-progeny population by crossing two globally diverse isolates, one from North Dakota, USA and the other from Western Australia. Progeny were phenotyped on a set of four barley genotypes showing a differential reaction to the parental isolates, then genotyped using a restriction-site associated – genotype by sequencing (RAD-GBS) approach. Genetic maps were developed for use in quantitative trait locus (QTL) analysis to identify virulence-associated QTL. Six QTL were identified on five different linkage groups and individually accounted for 20 to 37% of the disease variation with the number of significant QTL ranging from two to four for the barley genotypes evaluated. The data presented demonstrates the complexity of virluence involved in the P. teres f. maculata-barley interaction and begins to lay the foundation for understanding this important interaction.