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ARS Home » Midwest Area » Peoria, Illinois » National Center for Agricultural Utilization Research » Mycotoxin Prevention and Applied Microbiology Research » Research » Publications at this Location » Publication #337014
Research Project: Genomic and Metabolomic Approaches for Detection and Control of Fusarium, Fumonisins and Other Mycotoxins on Corn

Location: Mycotoxin Prevention and Applied Microbiology Research

Title: Resolving the Mortierellaceae phylogeny through Multi-Locus Sequence Typing (MLST) and phylogenomics

Author
item VANDE POL, NATALIE - Michigan State University
item STAJICH, JASON - University Of California
item O Donnell, Kerry
item DESIRO, ALESSANDRO - Michigan State University
item BONITO, GREGORY - Michigan State University

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 3/19/2017
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: The Mortierellaceae (Mortierellomycotina) are a diverse family of fungi that are of evolutionary and ecological relevance. They are the closest lineage to the arbuscular mycorrhizae (Glomeromycotina) and include some of the first species to evolve fruiting body production. The Mortierellaceae are estimated to contain at least 100 species classified within six polyphyletic genera that cannot be resolved with ribosomal markers. With advances in DNA sequencing technology, it is now feasible to generate sequence data from many loci in parallel (MLST), or to perform low-coverage genome (LCG) sequencing to identify phylogenetically informative loci. In collaboration with the ZyGo Life consortium and the Joint Genome Institute, we sequenced 68 LCGs representing 50 unique species of Mortierellaceae. From these, we identified 400 informative loci and used RaxML to build a concatenated tree. The resulting phylogeny has very strong bootstrap support and a very different structure from existing ribosomal trees. In parallel, we analyzed three de novo sequenced Mortierella genomes and extracted 13 informative loci. For each locus, we designed PCR primers for multiplexed PCR amplification across 333 Mortierellaceae isolates, which included the 68 LCG isolates. We will discuss the strengths and limitations of these two approaches.