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ARS Home » Pacific West Area » Pullman, Washington » WHGQ » Research » Publications at this Location » Publication #336679

Research Project: Improved Control of Stripe Rust in Cereal Crops

Location: Wheat Health, Genetics, and Quality Research

Title: Novel Sources of Stripe Rust Resistance Identified by Genome-Wide Association Mapping in Ethiopian Durum Wheat (Triticumturgidumssp. durum)

Author
item LIU, WEIZHEN - Washington State University
item MACAFERRI, MARCO - University Of Bologna, Italy
item RYNEARSON, SHERI - Washington State University
item LETTA, TESFAYE - Ethiopian Agricultural Research
item ZEGEYE, HABTEMARIAM - Ethiopian Institute Of Agricultural Research
item TUBEROSA, ROBERTO - University Of Bologna, Italy
item Chen, Xianming
item PUMPHREY, MICHAEL - Washington State University

Submitted to: Frontiers in Plant Science
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 12/30/2016
Publication Date: 12/30/2016
Citation: Liu, W., Macaferri, M., Rynearson, S., Letta, T., Zegeye, H., Tuberosa, R., Chen, X., Pumphrey, M. 2016. Novel Sources of Stripe Rust Resistance Identified by Genome-Wide Association Mapping in Ethiopian Durum Wheat (Triticumturgidumssp. durum). Frontiers in Plant Science. 8:774(1-15).

Interpretive Summary: Stripe rust of wheat is a global concern for wheat production. As Ethiopia has a long history of stripe rust epidemics, its native wheat germplasm harbors potentially valuable resistance genes. The objective of this study was to identify genes or loci conferring resistance to predominant races of the stripe rust pathogen in Ethiopia and the United States. Using a high-density 90K wheat single nucleotide polymorphism (SNP) array, a genome-wide association analysis (GWAS) was conducted on 182 durum wheat landrace accessions and contemporary varieties originating from Ethiopia. Landraces were detected to be more resistant at the seedling stage while cultivars were more resistant at the adult-plant stages. GWAS identified 68 loci associated with seedling resistance to one or more races. Six loci on chromosome arms 1AS, 1BS, 3AS, 4BL and 5BL were associated with resistance against at least two races at the seedling stage, and five loci were previously undocumented. GWAS analysis of field resistance reactions identified 12 loci associated with resistance on chromosomes 1A, 1B, 2B, 3B, 4A, 4B and 5A, which were detected in at least two of six field nurseries. Comparison with previously mapped resistance loci indicates that six of the 12 resistance loci are newly documented. The effective sources of resistance in Ethiopian durum wheat landraces can be transferred into adapted cultivars to provide resistance against stripe rust.

Technical Abstract: Stripe rust of wheat, caused by Puccinia striiformis f. sp. tritici (Pst), is a global concern for wheat production and has been increasingly destructive in Ethiopia,as well as in the United States and many other countries. As Ethiopia has a long history of stripe rust epidemics, its native wheat germplasm harbors potentially valuable resistance loci. Moreover, the Ethiopian germplasm has been historically underutilized in breeding of modern wheat worldwide and thus the resistant alleles from the Ethiopian germplasm represent potentially novel sources of biotic and abiotic stresses. The objective of this study was to identify loci conferring resistance to predominant Pst races in Ethiopia and the United States. Using a high-density 90K wheat single nucleotide polymorphism (SNP) array, a genome-wide association analysis (GWAS) was conducted on 182 durum wheat landrace accessions and contemporary varieties originating from Ethiopia. Landraces were detected to be more resistant at the seedling stage while cultivars were more resistant at the adult-plant stages. GWAS identified 68 loci associated with seedling resistance to one or more races. Six loci on chromosome arms 1AS, 1BS, 3AS, 4BL and 5BL were associated with resistance against at least two races at the seedling stage, and five loci were previously undocumented. GWAS analysis of field resistance reactions identified 12 loci associated with resistance on chromosomes 1A, 1B, 2B, 3B, 4A, 4B and 5A, which were detected in at least two of six field screening nurseries at the adult-plant stage. Comparison with previously mapped resistance loci indicates that six of the 12resistance loci are newly documented. This study report effective sources of resistance to contemporary races in Ethiopia and the United States and reveals that Ethiopian durum wheat landraces are abundant in novel Pst resistance loci that may be transferred into adapted cultivars to provide resistance against Pst.