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ARS Home » Plains Area » College Station, Texas » Southern Plains Agricultural Research Center » Food and Feed Safety Research » Research » Publications at this Location » Publication #331742

Research Project: Investigation of Immunoregulation in Reducing Foodborne Pathogen Colonization in Poultry

Location: Food and Feed Safety Research

Title: Chicken macrophages infected with Salmonella (S.) Enteritidis or S. Heidelberg produce differential responses in immune and metabolic signaling pathways

Author
item He, Louis - Haiqi
item Arsenault, Ryan - University Of Delaware
item Genovese, Kenneth - Ken
item Johnson, Casey - University Of Delaware
item Kogut, Michael - Mike

Submitted to: Veterinary Immunology and Immunopathology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 11/1/2017
Publication Date: 1/1/2018
Citation: He, L.H., Arsenault, R.J., Genovese, K.J., Johnson, C., Kogut, M.H. 2018. Chicken macrophages infected with Salmonella (S.) Enteritidis or S. Heidelberg produce differential responses in immune and metabolic signaling pathways. Veterinary Immunology and Immunopathology. 195:46-55. https://doi.org/10.1016/j.vetimm.2017.11.002.
DOI: https://doi.org/10.1016/j.vetimm.2017.11.002

Interpretive Summary: Salmonella are the most common foodborne pathogens from poultry and are often associated with human salmonellosis. Macrophage cells are one of the white blood cells that can produce chemicals to kill bacteria. Protein kinases are molecules that are very important for maintaining normal function of macrophage and for immune protection against pathogens. However, Salmonella are able to make changes to these kinases and survive inside the chicken macrophage cells. In this study, we used a tool called peptide array to study what kinases are changed in the chicken macrophage cells when infected by Salmonella. From this study we have found that Salmonella infection causes significant changes in hundreds of protein kinases and affects many cellular functions. This information helps us to better understand the mechanism of Salmonella infection and survival in chicken macrophage cells. Our findings are important to the pharmaceutical and poultry industries in the United States because they offer new knowledge to control Salmonella in poultry.

Technical Abstract: Protein kinases act in coordination with phosphatases to control protein phosphorylation and regulate signaling pathways and cellular processes involved in nearly every functions of cell life. Salmonella are known to manipulate the host kinase network to gain entrance and survive inside host cells. Effect of Salmonella infection on host kinase network has been studied in mammalian cells, but information is largely lacking in chicken immune cells. Although chicken macrophages are equipped with effective antimicrobial mechanisms, many Salmonella strains are able to survival inside the macrophage. In order to better understand the interaction between chicken macrophages and Salmonella, we used a peptide array-based kinome analysis to identify cellular process and signaling pathways that may play a critical role in the outcome of Salmonella infection. The kinome assay was performed on chicken HD-11 macrophages collected at 1.5, 3, and 7 h post-infection (hpi) with either S. Heidelberg or S. Enteritidis. A large number of peptides show significantly changed phosphorylation (p is less than or equal to 0.05) during the infection: 390, 449, and 575 peptides for S. Enteritidis and 185, 470, and 442 for S. Heidelberg at 1.5, 3, and 7 hpi, respectively. Many pathways involved in immunity, signal transduction, cellular process, and metabolism were significantly altered, in some case differentially, during the infection by the two Salmonella strains. Particularly, effects on lysosome process, iNOS, CARD9, NLRP3 inflammasome, MAPK p38, and mTOR provide significant insight to the interplay between pathogens and chicken macrophage cells during the infection.