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ARS Home » Plains Area » Fargo, North Dakota » Edward T. Schafer Agricultural Research Center » Cereal Crops Research » Research » Publications at this Location » Publication #331026

Research Project: HOST-PATHOGEN INTERACTIONS IN BARLEY AND WHEAT

Location: Cereal Crops Research

Title: Genetic analysis of net form net blotch resistance in barley lines CIho 5791 and Tifang against a global collection of P.teres f. teres isolates

Author
item Koladia, V - North Dakota State University
item Faris, Justin
item Richards, J - North Dakota State University
item Brueggeman, R - North Dakota State University
item Chao, Shiaoman
item Friesen, Timothy

Submitted to: Theoretical and Applied Genetics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 9/26/2016
Publication Date: 10/12/2016
Publication URL: http://handle.nal.usda.gov/10113/5695382
Citation: Koladia, V.M., Faris, J.D., Richards, J.K., Brueggeman, R.S., Chao, S., Friesen, T.L. 2016. Genetic analysis of net form net blotch resistance in barley lines CIho 5791 and Tifang against a global collection of P.teres f. teres isolates. Theoretical and Applied Genetics. 130:163-173. doi: 10.1007/s00122-016-2801-4.

Interpretive Summary: Net form net blotch NFNB is caused by the necrotrophic fungal pathogen Pyrenophora teres f. teres. The barley line CI5791 confers high levels of NFNB, with few documented cases of this resistance being overcome. Tifang barley also harbors resistance to P. teres f. teres which was previously shown to localize to barley chromosome 3H. A CI5791 × Tifang mapping population was developed and evaluated for NFNB resistance using nine P. teres f. teres isolates collected globally. Tifang was resistant to four of the isolates tested whereas CI5791 was highly resistant to all nine isolates. CI5791 resistance mapped to chromosome 6H whereas the Tifang resistance mapped to chromosome 3H. Additionally, CI5791 also harbored resistance at the same 3H region that was effective against two Japanese isolates. Molecular markers and mapping population lines harboring both 3H and 6H resistance can now be used in barley breeding programs.

Technical Abstract: The barley line CI5791 confers high levels of resistance to Pyrenophora teres f. teres, causal agent of net form net blotch (NFNB), with few documented isolates overcoming this resistance. Tifang barley also harbors resistance to P. teres f. teres which was previously shown to localize to barley chromosome 3H. A CI5791 × Tifang F6 recombinant inbred line (RIL) population was developed using single seed descent. The Illumina iSelect SNP platform was used to identify 2,562 single nucleotide polymorphism (SNP) markers across the barley genome, resulting in seven linkage maps, one for each barley chromosome. The CI5791 × Tifang RIL population was evaluated for NFNB resistance using nine P. teres f. teres isolates collected globally. Tifang was resistant to four of the isolates tested whereas CI5791 was highly resistant to all nine isolates. QTL analysis indicated that the CI5791 resistance mapped to chromosome 6H whereas the Tifang resistance mapped to chromosome 3H. Additionally, CI5791 also harbored resistance to two Japanese isolates that mapped to a 3H region similar to that of Tifang. SNP markers and RILs harboring both 3H and 6H resistance will be useful in resistance breeding against NFNB.