Location: Subtropical Plant Pathology ResearchTitle: Development and evaluation of ELISA and qRT-PCR for identification of Squash vein yellowing virus in cucurbits
|WEBSTER, C. - Former ARS Employee|
|LI, W. - University Of Florida|
|Kousik, Chandrasekar - Shaker|
Submitted to: Plant Disease
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 9/14/2016
Publication Date: 1/1/2017
Citation: Webster, C.G., Turechek, W., Li, W., Kousik, C.S., Adkins, S.T. 2017. Development and evaluation of ELISA and qRT-PCR for identification of Squash vein yellowing virus in cucurbits. Plant Disease. 101(1):178-185. https://doi.org/10.1094/PDIS-06-16-0872-RE.
Interpretive Summary: New methods were developed and evaluated for the identification of Squash vein yellowing virus (SqVYV), the cause of viral watermelon vine decline. The methods were capable of detecting SqVYV in multiple host plants. These new methods will assist in improved management of SqVYV-induced watermelon vine decline.
Technical Abstract: Enzyme linked-immunosorbent assay (ELISA) and quantitative reverse transcription-PCR (qRT-PCR) assays were developed for identification of Squash vein yellowing virus (SqVYV), the cause of viral watermelon vine decline. Both assays were capable of detecting SqVYV in a wide range of cucurbit hosts. The sensitivity and specificity for each procedure was estimated by a latent class analysis under a range of test conditions. qRT-PCR consistently showed sensitivities =0.99, whereas the sensitivity for ELISA ranged from 0.58-0.76. The newly developed diagnostic tools provide more options for the detection of SqVYV.