Location: Sugarcane ResearchTitle: Identification of quantitative trait loci controlling sucrose content based on an enriched genetic linkage map of sugarcane (saccharum spp. hybrids) cultivar ‘LCP 85-384’
|LIU, PINGWU - Guangxi University|
|CHANDRA, AMARESH - Indian Institute Of Sugarcane Research|
|QUE, YOUXIONG - Fujian Agricultural & Forestry University|
|CHEN, PING-HUA - Fujian Agricultural & Forestry University|
Submitted to: Plant and Animal Genome Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 10/19/2015
Publication Date: 1/9/2016
Citation: Pan, Y-B., Liu, P., Chandra, A., Que, Y., Chen, P., Grisham, M.P. 2016. Identification of quantitative trait loci controlling sucrose content based on an enriched genetic linkage map of sugarcane (saccharum spp. hybrids) cultivar ‘LCP 85-384’ [abstract]. International Plant and Animal Genome Conference, January 9-13, 2016, San Diego, CA.
Technical Abstract: Sucrose content is the most important trait in sugarcane breeding. Since sugarcane cultivars possess 100 to 130 chromosomes and are genetically complex polyploids and aneuploids, identification of quantitative trait loci (QTLs) associated with sucrose content is considered the best option to improve sucrose content through molecular breeding. A preliminary genetic linkage map of Louisiana sugarcane cultivar ‘LCP 85-384’ from a previous study was enriched using 65 additional polymorphism simple-sequence-repeats (SSR) primer-pairs to identify more co-segregated and homologous groups (CGs and HGs) and QTLs controlling sucrose content. Eighty-four SSR primer pairs produced 456 markers, of which 441 were polymorphic. Both simplex (993) and duplex (225) SSR, AFLP, and TRAP markers were included in the construction of the enriched LCP 85-384 map. These simplex and duplex markers were assigned to 108 CGs using JoinMap (v4.0). This map had a cumulative genome length of 7406.3 cM that included 675 AFLP (69.8 %), 90 TRAP (9.3 %), and 202 SSR (20.9 %) markers. The 202 SSR markers were assigned to 65 CGs and 8 HGs. Based on this map, 24 putative QTLs affecting sucrose content were identified. Five QTLs were unlinked and the other 19 QTLs were located on nine CGs within four HGs. Of these QTLs, 11 had an effect in both plant-cane (20.33 %) and first-stubble (25.68 %) crops. A higher efficiency of QTLs’ identification with AFLP, TRAP and SSR markers in such a genetically complex crop, proposes its wider utility in molecular breeding in sugarcane.