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ARS Home » Northeast Area » Beltsville, Maryland (BHNRC) » Beltsville Human Nutrition Research Center » Food Composition and Methods Development Laboratory » Research » Publications at this Location » Publication #320639

Research Project: Metabolite Profiling and Chemical Fingerprinting Methods for Characterization of Foods, Botanical Supplements, and Biological Materials

Location: Food Composition and Methods Development Laboratory

Title: Critical ratios for structural analysis of triacylglycerols using mass spectrometry

Author
item Byrdwell, W Craig

Submitted to: Lipid Technology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 9/15/2015
Publication Date: 12/31/2015
Citation: Byrdwell, W.C. 2015. Critical ratios for structural analysis of triacylglycerols using mass spectrometry. Lipid Technology. 27:11.

Interpretive Summary: Recent developments have finally allowed fragment behaviors using APCI-MS to be elucidated after twenty years of literature reports. Critical Ratios have been defined that correspond to various aspects of triacylglycerol (TAG) analysis, from overall degree of unsaturation to localization of fatty acids on the glycerol backbone (regioisomers), to grouping of unsaturated fatty acids. The same ratios also constitute a compact library of TAG mass spectra.

Technical Abstract: Recent developments have finally allowed fragment behaviors using APCI-MS to be elucidated after twenty years of literature reports. Critical Ratios have been defined that correspond to various aspects of triacylglycerol (TAG) analysis, from overall degree of unsaturation to localization of fatty acids on the glycerol backbone (regioisomers), to grouping of unsaturated fatty acids. The same ratios also constitute a compact library of TAG mass spectra.