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Research Project: Genetic Improvement of Small Grains for Biotic and Abiotic Stress Tolerance and Characterization of Pathogen Populations

Location: Plant Science Research

Title: Mapping of Fusarium Head Blight resistance QTL in winter wheat cultivar NC-Neuse

Author
item PETERSON, STINE - North Carolina State University
item LYERLY, JEANETTE - North Carolina State University
item MALONEY, PETER - North Carolina State University
item Brown-Guedira, Gina
item Cowger, Christina
item Costa, Jose
item DONG, YANHONG - University Of Minnesota
item MURPHY, J - North Carolina State University

Submitted to: Crop Science
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 3/3/2016
Publication Date: 5/27/2016
Citation: Peterson, S., Lyerly, J., Maloney, P., Brown Guedira, G.L., Cowger, C., Costa, J., Dong, Y., Murphy, J.P. 2016. Mapping of Fusarium Head Blight resistance QTL in winter wheat cultivar NC-Neuse. Crop Science. 56(4):1473-1483.

Interpretive Summary: Fusarium Head Blight (FHB) can significantly reduce the grain quality of wheat due to mycotoxin contamination. The objective of this study was to identify DNA markers linked to genes for FHB resistance in the moderately resistant soft red winter wheat cultivar NC-Neuse using a population developed from a cross between NC-Neuse and the susceptible cultivar AGS 2000. A total of 170 progeny were evaluated following inoculation with F. graminearum infested corn kernels in mist-irrigated field nurseries. The lines were evaluated for disease incidence (INC), severity (SEV), Fusarium-damaged kernels (FDK), and DON content (DON) in seven environments between 2011 and 2014. A linkage map was developed based on 1839 simple sequence repeat DNA markers. Analyses identified seven FHB resistance loci on wheat chromosomes 1A, 1B, 1D, 2A, 4A, 5B, and 6A. These genes reduced disease from 5.9 % for SEV to 19.5% for FDK. High throughput assays were developed for each NC-Neuse QTL region. A preliminary test using these assays on recent Uniform Southern Winter Wheat Nursery entries indicated loci on chromosomes 1A and 6A as likely the best candidates for use in marker-assisted selection.

Technical Abstract: Fusarium Head Blight (FHB), primarily caused by Fusarium graminearum, can significantly reduce the grain quality of wheat (Triticum aestivum L.) due to mycotoxin contamination. The objective of this study was to identify quantitative trait loci (QTL) for FHB resistance in the moderately resistant soft red winter wheat cultivar NC-Neuse using a population developed from a cross between NC-Neuse and the susceptible cultivar AGS 2000. A total of 170 recombinant inbred lines (RILs) were evaluated following inoculation with F. graminearum infested corn kernels in mist-irrigated field nurseries. The lines were evaluated for disease incidence (INC), severity (SEV), Fusarium-damaged kernels (FDK), and DON content (DON) in seven environments between 2011 and 2014. A 3,419 cM linkage map was developed based on 1839 simple sequence repeat (SSR), diversity array technology (DArT), and single nucleotide polymorphism (SNP) markers. Composite Interval Mapping (CIM) and Multiple Interval Mapping (MIM) analyses for adjusted means within and across environments identified seven FHB resistance QTL on chromosomes 1A, 1B, 1D, 2A, 4A, 5B, and 6A. The QTL alleles conferring resistance on 1A, 1B, 2A, 4A, and 6A originated from NC-Neuse. The QTL alleles associated with resistance on chromosomes 1D and 5B originated from AGS 2000. QTL effects ranged from 9.3 to 11.7% for INC, from 5.9 to 10.7% for SEV, from 7.8 to 19.5% for FDK, and from 5.7 to 18.2% for DON. At the QTL on chromosome 1B, susceptibility was associated with the 1BL.1RS wheat-rye translocation from AGS 2000. The QTL on 5B co-localized with the Vrn-B1 locus. Kompetitive Allele-Specific PCR (KASP) assays were developed for each NC-Neuse QTL region. A preliminary test using these assays on recent Uniform Southern Winter Wheat Nursery (USWWN) entries indicated Qfhb.nc-1A and Qfhb.nc-6A as likely the best candidates for use in marker-assisted selection.