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ARS Home » Pacific West Area » Pullman, Washington » WHGQ » Research » Publications at this Location » Publication #318249

Research Project: Improved Control of Stripe Rust in Cereal Crops

Location: Wheat Health, Genetics, and Quality Research

Title: Virulence and SSR marker segregation in a Puccinia striiformis f. sp. tritici population produced by selfing a Chinese isolate on Berberis shensiana

Author
item TIAN, Y. - Northwest Agriculture And Forestry University
item ZHAN, G. - Northwest Agriculture And Forestry University
item Chen, Xianming
item TUNGRUENTRAGOON, A. - Northwest Agriculture And Forestry University
item LU, X. - Northwest Agriculture And Forestry University
item ZHAO, J. - Northwest Agriculture And Forestry University
item HUANG, L. - Northwest Agriculture And Forestry University
item KANG, Z. - Northwest Agriculture And Forestry University

Submitted to: Phytopathology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 10/3/2015
Publication Date: 2/1/2016
Citation: Tian, Y., Zhan, G.M., Chen, X., Tungruentragoon, A., Lu, X., Zhao, J., Huang, L.L., Kang, Z.S. 2016. Virulence and SSR marker segregation in a Puccinia striiformis f. sp. tritici population produced by selfing a Chinese isolate on Berberis shensiana. Phytopathology. 106:(2):185-191.

Interpretive Summary: The wheat stripe rust pathogen, which has a complete asexual and sexual life cycle including five spore stages, is highly variable. The fungal pathogen produces new races overcoming resistance in wheat cultivars. A recently identified race, V26 with virulence to resistance gene Yr26 and many other stripe rust resistance genes, has a high potential to cause epidemics in China and other countries. In this study, teliospores from a single-urediniospore isolate of V26 produced on a Yr26 wheat line were used to produce a sexual population through selfing by infecting barberry plants under controlled conditions. One hundred and eighteen progeny isolates and the parental isolate were phenotyped for virulence/avirulence on 24 resistance gene lines of wheat. The progeny isolates were all avirulent to Yr5, Yr8, Yr15, and YrTr1; and virulent to Yr1, Yr2, Yr7, Yr9, Yr10, Yr17, Yr24, Yr25, Yr26, YrA, YrExp2, andYrV23, indicating that the parental isolate is homozygous avirulent or homozygous virulent at these loci. Virulences to different resistance genes were either dominant or receive, and controlled by either one gene ot two genes. From the 118 progeny isolates, 28 races were identified. From the 118 progeny isolates, 82 multilocus genotypes were identified. The results show that a highly diverse population can be produced from a single isolate by selfing on a barberry plant and sexually produced population can be used to genetically characterize virulence of the stripe rust pathogen.

Technical Abstract: Puccinia striiformis f. sp. tritici (Pst), the causal agent of wheat stripe rust, is highly variable. The fungal pathogen produces new races overcoming resistance in wheat cultivars. A recently identified race, V26 with virulence to Yr26 and many other stripe rust resistance genes, has a high potential to cause epidemics in China. In this study, teliospores from a single-urediniospore isolate of V26 (No. Pinglan 17-7) produced on the wheat line 92R137 (Yr26) were used to produce a sexual population through selfing by infecting Berberis shensiana plants under controlled conditions. One hundred and eighteen progeny isolates and the parental isolate were phenotyped for virulence/avirulence on 24 Yr gene lines of wheat. These progeny isolates were all avirulent to Yr5, Yr8, Yr15, and YrTr1; and virulent to Yr1, Yr2, Yr7, Yr9, Yr10, Yr17, Yr24, Yr25, Yr26, YrA, YrExp2, andYrV23,indicating that the parental isolate is homozygous avirulent or homozygous virulent at these loci. The progeny population segregated for avirulences to Yr6 and Yr43 at one locus (3 avirulent : 1 virulent isolates) and YrSP at two loci (13 avirulent : 3 virulent isolates); for virulence to Yr27 and Yr28 (3 virulent : 1 avirulent) at one locus; and for Yr4, Yr32, and Yr44 (15 virulent : 1 avirulent) at two loci. From the 118 progeny isolates, 28 pathotypes were identified. Among the eight segregating avirulence/virulence loci, virulence genes to Yr4 and Yr32 were found associated and those to Yr6 and Yr43 were associated based Chi-squared tests. From the 118 progeny isolates, 82 multilocus genotypes were identified. The results show that a highly diverse population can be produced from a single isolate by selfing on a barberry plant and sexually produced population can be used to genetically characterize virulence of the stripe rust pathogen.