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ARS Home » Pacific West Area » Pullman, Washington » WHGQ » Research » Publications at this Location » Publication #317409

Research Project: Improved Control of Stripe Rust in Cereal Crops

Location: Wheat Health, Genetics, and Quality Research

Title: Characterization and molecular mapping of stripe rust resistance gene Yr61 in winter wheat cultivar Pindong 34

Author
item ZHOU, X - Northwest Agriculture And Forestry University
item HAN, D - Northwest Agriculture And Forestry University
item Chen, Xianming
item GOU, H - Northwest Agriculture And Forestry University
item GUO, S - Northwest Agriculture And Forestry University
item RONG, L - Northwest Agriculture And Forestry University
item WANG, Q - Northwest Agriculture And Forestry University
item HUANG, L - Northwest Agriculture And Forestry University
item KANG, Z - Northwest Agriculture And Forestry University

Submitted to: Theoretical and Applied Genetics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 8/15/2014
Publication Date: 10/27/2014
Citation: Zhou, X.L., Han, D.J., Chen, X., Gou, H.L., Guo, S.J., Rong, L., Wang, Q.L., Huang, L.L., Kang, Z.S. 2014. Characterization and molecular mapping of stripe rust resistance gene Yr61 in winter wheat cultivar Pindong 34. Theoretical and Applied Genetics. 127(11):2349-2358.

Interpretive Summary: Several new races of the stripe rust pathogen have established throughout the wheat growing regions of China in recent years. These new races are virulent to most of the designated seedling resistance genes limiting the resistance sources. It is necessary to identify new genes for diversification and for pyramiding different resistance genes in order to achieve more durable resistance. We report here the identification of a new resistance gene, designated as Yr61, in Chinese wheat cultivar Pindong 34. A mapping population developed from a cross between Mingxian 169 and Pindong 34 was evaluated for seedling stripe rust response. A genetic map consisting of fourteen molecular markers was constructed. The resistance gene was located on the short arm of chromosome 7A and flanked by two markers about 1.9 and 3.9 cM in distance. The two flanking markers were successfully converted into two easier-to-use markers, which could be used for marker-assisted selection of the stripe rust resistance gene in breeding programs.

Technical Abstract: Several new races of the stripe rust pathogen have established throughout the wheat growing regions of China in recent years. These new races are virulent to most of the designated seedling resistance genes limiting the resistance sources. It is necessary to identify new genes for diversification and for pyramiding different resistance genes in order to achieve more durable resistance. We report here the identification of a new resistance gene, designated as Yr61, in Chinese wheat cultivar Pindong 34. A mapping population of 208 F2 plants and 128 derived F2:3 lines in a cross between Mingxian 169 and Pindong 34 was evaluated for seedling stripe rust response. A genetic map consisting of eight resistance gene analog polymorphism (RGAP), two sequence-tagged site (STS) and four simple sequence repeat (SSR) markers was constructed. Yr61 was located on the short arm of chromosome 7A and flanked by RGAP markers Xwgp5467 and Xwgp5765 about 1.9 and 3.9 cM in distance, which were successfully converted into STS markers STS5467 and STS5765b, respectively. The flanking STS markers could be used for marker-assisted selection of Yr61 in breeding programs.