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ARS Home » Pacific West Area » Pullman, Washington » WHGQ » Research » Publications at this Location » Publication #317407

Research Project: Improved Control of Stripe Rust in Cereal Crops

Location: Wheat Health, Genetics, and Quality Research

Title: Multi-location wheat stripe rust QTL analysis: genetic background and epistatic interactions

Author
item VAZQUEZ, M - Oregon State University
item ZEMETRA, R - Oregon State University
item PETERSON, C - Limagrain Cereal Seeds
item Chen, Xianming
item HEESACKER, A - Oregon State University
item MUNDT, C - Oregon State University

Submitted to: PLoS ONE
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 3/20/2015
Publication Date: 4/7/2015
Citation: Vazquez, M.D., Zemetra, R., Peterson, C.J., Chen, X., Heesacker, A., Mundt, C.C. 2015. Multi-location wheat stripe rust QTL analysis: genetic background and epistatic interactions. PLoS One. 128(7):1307-1318. doi: 10.1007/s00122-015-2507-z.

Interpretive Summary: Stripe rust is a foliar disease of wheat caused by the air-borne stripe rust fungus and is present in most regions around the world where commercial wheat is grown. Breeding for durable resistance to stripe rust continues to be a priority, but also is a challenge due to the complexity of interactions among resistance genes and to the wide diversity and continuous evolution of the pathogen races. The goal of this study was to detect chromosomal regions for resistance to stripe rust in two winter wheat populations, ‘Tubbs’/‘NSA-98-0995’ (T/N) and ‘Einstein’/‘Tubbs’ (E/T), evaluated across seven environments and genetically mapped with diversity array technology and simple sequence repeat markers. Analysis of variance for phenotypic data revealed significant genotypic differentiation for stripe rust among the recombinant inbred lines. Results for quantitative trait locus analysis in the E/T population indicated that two major loci located in chromosomes 2AS and 6AL were responsible for the main phenotypic response. For the T/N population, eight loci were found; chromosomes 2AL and 2BL accounting for most of the phenotypic variance. The results are useful for developing stripe rust resistant wheat varieties.

Technical Abstract: Stripe rust is a foliar disease of wheat (Triticum aestivum L.) caused by the air-borne fungus Puccinia striiformis f. sp. tritici and is present in most regions around the world where commercial wheat is grown. Breeding for durable resistance to stripe rust continues to be a priority, but also is a challenge due to the complexity of interactions among resistance genes and to the wide diversity and continuous evolution of the pathogen races. The goal of this study was to detect chromosomal regions for resistance to stripe rust in two winter wheat populations, ‘Tubbs’/‘NSA-98-0995’ (T/N) and ‘Einstein’/‘Tubbs’ (E/T), evaluated across seven environments and mapped with diversity array technology and simple sequence repeat markers covering polymorphic regions of ˜1480 and 1117 cM, respectively. Analysis of variance for phenotypic data revealed significant (P < 0.01) genotypic differentiation for stripe rust among the recombinant inbred lines. Results for quantitative trait loci/locus (QTL) analysis in the E/T population indicated that two major QTL located in chromosomes 2AS and 6AL, with epistatic interaction between them, were responsible for the main phenotypic response. For the T/N population, eight QTL were identified, with those in chromosomes 2AL and 2BL accounting for the largest percentage of the phenotypic variance.