|BADILLO-VARGAS, ISMAEL - University Of Florida|
|BAKER, CARLYE - Florida Department Of Agriculture And Consumer Services|
|FUNDERBURK, JOE - University Of Florida|
Submitted to: Thysanoptera International Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 3/24/2015
Publication Date: N/A
Technical Abstract: A novel plant virus has been identified infecting fresh market tomato plants in south and southeast Florida. The virus causes necrosis of tomato leaves, petioles and stems, and necrotic rings or spots on tomato fruits. Symptomatic tomato plant tissue was used to mechanically inoculate tomato plants and reproduced symptoms observed in the original field samples. A portion of the replicase protein 1a gene from RNA1, and the complete movement protein (MP) and coat protein (CP) genes from RNA3 were amplified and sequenced. Sequence analysis showed that these three viral genes are most closely related to, but distinct from, Tulare apple mosaic virus and other subgroup 2 ilarviruses. Considerably less identity was observed with previously reported subgroup 1 tomato-infecting ilarviruses including Tobacco streak virus, Parietaria mottle virus, and Tomato necrotic spot virus. These results indicate that the tomato symptoms observed in Florida in fall 2013, and subsequently in spring and fall 2014, are caused by a novel subgroup 2 ilarvirus, for which the name Tomato necrotic streak virus (TomNSV) is proposed. Potential TomNSV transmission by pollen, seed and/or insect vectors, which may include thrips similar to other ilarviruses, is being investigated.