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ARS Home » Midwest Area » St. Paul, Minnesota » Plant Science Research » Research » Publications at this Location » Publication #315578

Research Project: Enhanced Alfalfa Germplasm and Genomic Resources for Yield, Quality, and Environmental Protection

Location: Plant Science Research

Title: Complete genome sequence of Clavibacter michiganensis subsp. insidiosus R1-1 using PacBio single-molecule real-time technology

Author
item LU, YOU - University Of Minnesota
item Samac, Deborah - Debby
item GLAZEBROOK, JANE - University Of Minnesota
item ISHIMARU, CAROL - University Of Minnesota

Submitted to: Genome Announcements
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 3/23/2015
Publication Date: 5/7/2015
Publication URL: http://handle.nal.usda.gov/10113/60879
Citation: Lu, Y., Samac, D.A., Glazebrook, J., Ishimaru, C.A. 2015. Complete genome sequence of Clavibacter michiganensis subsp. insidiosus R1-1 using PacBio single-molecule real-time technology. Genome Announcements. 3:e00396-15.

Interpretive Summary: Bacterial wilt of alfalfa occurs throughout most alfalfa growing regions worldwide. For this reason the pathogen is considered a phytosanitary risk for international seed movement. The lack of a complete genome sequence for the pathogen causing this disease has hampered progress in understanding pathogen biology. The complete genome sequence was obtained using two sequencing methods. This resulted in very high quality sequence information. A total of 3,317 protein-coding genes were identified. Availability of the complete genome sequence will be valuable for developing molecular diagnostic tools and understanding the mechanisms the pathogen uses for causing disease.

Technical Abstract: We report the complete genome sequence of Clavibacter michiganensis subsp. insidiosus R1-1 isolated in Minnesota, USA. The R1-1 genome, generated by de novo assembly of PacBio sequencing data, is the first complete genome sequence available for this subspecies.