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ARS Home » Southeast Area » Athens, Georgia » U.S. National Poultry Research Center » Exotic & Emerging Avian Viral Diseases Research » Research » Publications at this Location » Publication #311574

Title: Virus isolation and propagation in embryonating eggs

item Spackman, Erica
item STEPHENS, CHRISTOPHER - University Of Georgia

Submitted to: Isolation and Identification of Avian Pathogens
Publication Type: Book / Chapter
Publication Acceptance Date: 3/1/2015
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: The embryonating egg is one of the most versatile, easy to work with, and widely used host systems for the isolation and propagation of avian viruses. The embryonating chicken egg (ECE) is the most commonly available system that is both specific pathogen free and supports the replication of viruses from many species. ECE can be inoculated by numerous routes and can accommodate most sample types, such as swabs and tissues. Fluids, membranes, and organs can be harvested from ECE, which can then be used as a high titered source of virus. Also, the embryo itself can be directly examined for gross lesions to assist with diagnosis.