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ARS Home » Southeast Area » Dawson, Georgia » National Peanut Research Laboratory » Research » Publications at this Location » Publication #310605

Research Project: Genetics, Populations Dynamics, and Mycotoxin Prevention in Peanut

Location: National Peanut Research Laboratory

Title: Environmentally friendly and cost-efficient analysis of aflatoxins in corn

Author
item Sobolev, Victor

Submitted to: World Mycotoxins Forum
Publication Type: Abstract Only
Publication Acceptance Date: 9/1/2014
Publication Date: 11/10/2014
Citation: Sobolev, V. 2014. Environmentally friendly and cost-efficient analysis of aflatoxins in corn. World Mycotoxins Forum 8th Conference.

Interpretive Summary: The analysis of natural toxins in agricultural commodities includes their extraction with organic solvents. Existing extraction methods are time-consuming and expensive. An inexpensive and low-waste extraction method using a household espresso coffee maker was tested. The method demonstrated high recovery of the carcinogenic aflatoxins from corn samples, good reproducibility and accuracy.

Technical Abstract: The extraction procedure adds a significant cost to the overall expense of aflatoxin analysis in agricultural commodities. An inexpensive and low-waste extraction method using a household espresso coffee maker was tested. This appliance was used for the high-temperature /high-pressure extraction of aflatoxins. The pump pressure of 18 kg/cm2 and water temperature close to boiling point make this device suitable for rapid extraction. A representative ground corn sample was mixed with diatomaceous earth and an organic water-miscible solvent followed by extraction with hot water in an espresso coffee maker. The extract was then analyzed for aflatoxins by HPLC. Recoveries of aflatoxins B1, B2, G1, and G2 from corn at 5 µg/kg were: 80.3±1.3, 89.1±4.8, 86.4±3.1, and 84.0±4.6%, respectively.