Skip to main content
ARS Home » Southeast Area » Houma, Louisiana » Sugarcane Research » Research » Publications at this Location » Publication #309345

Research Project: Genetic Improvement of Sugarcane for Temperate Climates

Location: Sugarcane Research

Title: Segregation analysis of microsatellite (SSR) markers in sugarcane polyploids

Author
item Pan, Yong-Bao
item LU, XIN - Yunnan Academy Of Agriculture Sciences
item ZHOU, HUI - Guangxi Academy Of Agricultural Sciences
item CHEN, CHARLES - Auburn University
item ZHU, JIAN-RONG - Yunnan Academy Of Agriculture Sciences
item CHEN, PING-HUA - Fujian Agricultural & Forestry University
item CAI, QING - Yunnan Academy Of Agriculture Sciences
item CHEN, RU-KAI - Fujian Agricultural & Forestry University

Submitted to: Plant and Animal Genome Conference
Publication Type: Abstract Only
Publication Acceptance Date: 9/10/2014
Publication Date: 1/12/2015
Citation: Pan, Y.-B., Lu, X., Zhou, H., Chen, C.Y., Zhu, J., Chen, P., Cai, Q., Chen, R. 2015. Segregation analysis of microsatellite (SSR) markers in sugarcane polyploids [abstract]. Plant and Animal Genome XXIII Conference. Paper No. 13970. Available: https://pag.confex.com/pag/xxiii/webprogram/Paper13970.html

Interpretive Summary:

Technical Abstract: Although the microsatellite (SSR) DNA markers have been extensively used in sugarcane breeding research, little is known about its inheritance mechanism. To address this problem, a high throughput molecular genotyping experiment was conducted on 964 single pollen grains and a 288-self progeny S1 mapping population of a Louisiana sugarcane cultivar LCP 85-384. Through PCR, five polymorphic SSR markers (SMC336BS, SMC1604SA, SCM7, UGSM10, and UGSM97) primed the amplification of 20 alleles. Segregation analysis of each allele among both pollen and S1 populations showed the following: 1) Inheritance of each SSR allele was in accordance with the Mendelian laws of segregation and independent assortment; 2) The polymorphism information content (PIC) values of the five SSR markers based on the pollen population were greater than those based on the S1 population, probably due to the sharing of same alleles between female and male gametes; 3)Fewer SSR genotypes were observed for each SSR marker from the S1 population than those from the pollen population; and 4) Only 11 out of the 20 SSR alleles (55%) segregated in accordance with Mendelian segregation ratios expected from S1 and pollen populations of a 2n = 10 polyploid. The results from this study will provide some guidance for sugarcane breeders in selecting crossing parents, estimating optimum size of seedling population, and promoting efficient selection.