Author
RAMESH, S.V. - Indian Council Of Agricultural Research (ICAR) | |
RAIKHY, G. - Washington State University | |
Brown, Charles | |
Whitworth, Jonathan | |
PAPPU, H.R. - Washington State University |
Submitted to: Archives of Virology
Publication Type: Peer Reviewed Journal Publication Acceptance Date: 8/25/2014 Publication Date: N/A Citation: N/A Interpretive Summary: Potato mop-top virus (PMTV) was first reported in the U.S. in Maine in 2002. Other reports from Canada and the U.S. have followed. PMTV is transmitted by the soil borne pathogen (Spongospera subterranean f.sp. Subterranea) that causes powdery scab in potato. A positive test for PMTV was obtained with an ELISA test from samples that showed concentric rings in the tubers. The samples were collected from a commercial potato growing region in Washington in the summer of 2013. Further characterization of the isolate was done by a complete sequence of the isolate and comparison with other sequences from North America and Europe. Results from phylogenetic analysis of the viral genes showed that the Washington isolate is closely related to the European isolates and is considered RNA TGB-II and RNA CP-B types, which are common in Europe and other areas of the world. Technical Abstract: Potato mop-top virus (PMTV) (family: Virgaviridae) was reported recently in the Pacific North-western USA. To better understand the genetic diversity of the virus, the complete genome of an isolate from Washington State (WA), USA was characterized. Sequence comparisons of the WA isolate with other known sequences revealed that the RNA Rep-encoded RdRp protein and the RNA CP-encoded coat protein displayed >99% amino acid identity to those of two Nordic (RdRp) and several European and North American isolates (CP) respectively. The RNA TGB-encoded TGB 1 and TGB 3 protein sequences had >99% amino acid identity to the corresponding proteins of the Czech and Danish isolates, whereas the TGB 2 protein is identical to the Colombian isolates. Phylogenetic analysis of the viral genes of the WA isolate reflected the close relationship among the WA and European isolates. RFLP analysis of corresponding DNA of RNA TGB and RNA CP revealed that WA isolate is considered RNA TGB-II and RNA CP-B types, which are prevalent in Europe and other parts of world. This is the first report of the complete genome characterization of PMTV from the Americas. |