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ARS Home » Plains Area » Fargo, North Dakota » Edward T. Schafer Agricultural Research Center » Insect Genetics and Biochemistry Research » Research » Publications at this Location » Publication #305958

Research Project: INSECT CRYOPRESERVATION, DORMANCY, GENETICS AND BIOCHEMISTRY

Location: Insect Genetics and Biochemistry Research

Title: 28S ribosomal RNA sequences separate five prominent Lygus (Hemiptera: Miridae) pest species into three species clu

Author
item Roehrdanz, Richard
item Sears Wichmann, Sheila

Submitted to: Entomological Society of America Annual Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 5/30/2014
Publication Date: 11/16/2014
Citation: Roehrdanz, R.L., Sears Wichmann, S.G. 2014. 28S ribosomal RNA sequences separate five prominent Lygus (Hemiptera: Miridae) pest species into three species clusters [abstract]. 2014 Entomological Society of America Annual Meeting. November 16-19, 2014. Portland, OR. Paper No. D3587.

Interpretive Summary: A segment of the 28S rRNA gene was compared among six species of Lygus (L. hesperus, L. keltoni, L. borealis, L. elisus, L. lineolaris, L. vanduzeii). The DNA sequences separate into three main groups. The LL group contains L. lineolaris and L. vanduzeii. Group LBLE is comprised of L. elisus and most of the L. borealis. Group LH includes L. hesperus and most of L. keltoni. Some L. keltoni were part of the LBLE group and some L. borealis were part of the LH group. The 28S region does not contain sufficient polymorphism to delineate species. The apparent polyphyly of L. borealis and L. keltoni could reflect historic interbreeding or highlight inadequacies of morphospecies identification.

Technical Abstract: A segment of the 28S rRNA gene was compared among six species of Lygus (L. hesperus, L. keltoni, L. borealis, L. elisus, L. lineolaris, L. vanduzeii). The DNA sequences separate into three main groups. The LL group contains L. lineolaris and L. vanduzeii. Group LBLE is comprised of L. elisus and most of the L. borealis. Group LH includes L. hesperus and most of L. keltoni. Some L. keltoni were part of the LBLE group and some L. borealis were part of the LH group. The 28S region does not contain sufficient polymorphism to delineate species. The apparent polyphyly of L. borealis and L. keltoni could reflect historic interbreeding or highlight inadequacies of morphospecies identification.