|WEN, GUOYUAN - Hubei Academy Of Agricultural Sciences|
Submitted to: Southeastern Regional Virology Conference
Publication Type: Abstract Only
Publication Acceptance Date: 4/10/2014
Publication Date: 4/25/2014
Citation: Wen, G., Yu, Q. 2014. A novel thermostable Newcastle disease virus vaccine vector for expression of a heterologous gene [abstract]. In: Proceedings of the Southeastern Regional Virology Conference, April 25-27, 2014, Atlanta, Georgia. p. 34.
Technical Abstract: The thermostable Newcastle disease virus (NDV) vaccines have been used widely to control Newcastle disease for village flocks, especially in the developing countries. To explore the potential use of the thermostable NDV as a vaccine vector, a reverse genetic system for the thermostable avirulent NDV strain TS09-C was established. The green fluorescence protein (GFP) gene, along with the self-cleaving foot-and-mouth virus 2A gene and Ubiquitin monomer (2AUbi), was inserted immediately upstream of the M gene translation start codon of NDV rTS09-C. The rescued virus rTS-GFP/M showed similar thermostability, growth dynamics, and pathogenicity as the parental rTS09-C virus. Both the rTS09-C and rTS-GFP/M viruses replicated efficiently in the BHK-21 cells in the absence of trypsin. Expression of the GFP in rTS-GFP/M-infected cells was detected by fluorescence microscopy and Western blot assay. Vaccination of specific pathogen free (SPF) chickens with the rTS-GFP/M virus conferred complete protection against velogenic NDV challenge. Taken together, the data demonstrated that the heterologous gene could be inserted and expressed in the thermostable NDV strain rTS09-C, suggesting that the rTS09-C virus can be used as a thermostable NDV vaccine vector to express an antigen of other avian pathogens as a novel bivalent vaccine against NDV and the target avian disease for backyard chickens.