Submitted to: Animal Genetics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 7/9/2014
Publication Date: 8/26/2015
Citation: Li, C., Beck, B.H., Fuller, S.A., Peatman, E. 2015. Transcriptome annotation and marker discovery in white bass (Morone chrysops) and striped bass (Morone saxatilis). Animal Genetics. 45(6):885-887.
Interpretive Summary: Hybrid striped bass, a cross between male striped bass and female white bass, are a major aquaculture species in the United States. However, compared to other important aquaculture species such as trout and catfish, genetic information for striped bass/white bass and their hybrid is currently lacking. A major area for improvement is a better understanding of the diversity of genes and their expression patterns in striped/white bass tissues. In the present study, we sequenced and identified thousands of different genes in numerous tissues of both striped and white bass. This information will function as a reference transcriptome, a valuable new tool that will enhance studies on reproduction, physiology, immunology, and broodstock selection in these commercially important farmed fish species.
Technical Abstract: Striped bass and white bass are the parental species of the hybrid striped bass (white bass, Morone chrysops X striped bass, M. saxatilis), which is a major U.S. aquaculture species. Currently, genomic resources for striped bass/white bass and its hybrid lag behind those of other aquaculture species. Current resources consist of a medium density genetic linkage map and a well-annotated ovarian transcriptome. A well-annotated transcriptome from across striped bass and white bass tissues is needed to advance both broad-based RNA-seq studies of gene expression as well as aid in more targeted studies of important genes and pathways. Here we carried out Illumina-based transcriptome sequencing in both species. The assembled Moronid reference transcriptomes and identified SSRs and SNPs should advance ongoing studies of reproduction, physiology, and immunology in these species and provide markers for broodstock selection.