|Johnson, Franklin - Washington State University Extension Service|
|Zheng, Ping - Washington State University Extension Service|
|Evans, Kate - Washington State University Extension Service|
|Main, Dorrie - Washington State University Extension Service|
Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 3/30/2014
Publication Date: 6/24/2014
Citation: Johnson, F., Zheng, P., Rudell Jr, D.R., Evans, K., Main, D., Zhu, Y. 2014. Profiling the transcriptomic and metabolomic changes associated with apple fruit controlled-atmosphere storage related peel disorder. Meeting Abstract. 79. Proceedings of 7th International Rosaceae Genomics Conference.
Technical Abstract: 'Golden Delicious' (Malus x domestica Borkh.) (GD) is one of the most widely available pome fruit. External CO2-injury, a postharvest controlled-atmosphere storage related peel disorder, significantly impacts long-term storability and fruit quality for fresh apple and pear fruit cultivars, although annual incidence varies greatly. The objective of this research was to understand the molecular responses of the peel tissue under controlled-atmosphere storage. The experiment included 5%CO2-controlled atmosphere (CA), 5%CO2-CA plus 1-methylcyclopropene (CA+1-MCP), and regular air (RA) treatments and peel tissues were sampled at 14, 28, 56, 84 days storage over a three year period. RNA-Seq based transcriptome profiling and lipidome profiling by LCMS were carried out for sampled apple peel tissues. KEGG pathway and Gene Ontology (GO) analyses indicated oxidation/reduction, glycolysis, Na2+ transporters and glutamate biosynthesis enriched in CA but not RA and CA+1-MCP. Accordingly, gene ontology for CA emphasized the importance of the endoplasmic reticulum (ER), ER stress and the Unfolded Protein Response while CA+1-MCP down regulated these ER stress response and protein folding genes. Multivariate and time course analysis of RNA-seq (2011) and LCMS/APCI (2011-2012) data provided genes and lipids of interest. SKP2A, an F-box protein known to be involved in severe ER stress, was detected only in CA stored fruit after 28 days storage. Results for the lipidome show (C18:3/18:2) diglyceride, ß-SitoGluclinolenate, two monogalactose-diacylglycerol and two triacylglycerols species having a significant differences between treatments. Highly relevant genes selected by sparse Partial Least Squares network analysis is being validated by qRT-PCR using all three years GD peel tissue.