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ARS Home » Northeast Area » Beltsville, Maryland (BARC) » Beltsville Agricultural Research Center » National Germplasm Resources Laboratory » Research » Publications at this Location » Publication #302787

Research Project: CHARACTERIZING, DETECTING, AND ELIMINATING PATHOGENS TO ENABLE THE SAFE INTRODUCTION OF PLANT GENETIC RESOURCES

Location: National Germplasm Resources Laboratory

Title: Clematis Chlorotic Mottle Virus, a novel virus occurring in Clematis in the USA

Author
item Mollov, Dimitre
item Lockhart, Ben - University Of Minnesota
item Phibbs, Anette - Wisconsin Department Of Agriculture
item Creswell, Tom - Purdue University
item Ruhl, Gail - Purdue University
item Dorman, Elizabeth - Michigan Department Of Agriculture
item Kinard, Gary
item Jordan, Ramon - Us Department Of Agriculture (USDA)

Submitted to: American Phytopathological Society Annual Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 5/1/2014
Publication Date: 8/9/2014
Citation: Mollov, D.S., Lockhart, B., Phibbs, A., Creswell, T., Ruhl, G., Dorman, E., Kinard, G.R., Jordan, R. 2014. Clematis Chlorotic Mottle Virus, a novel virus occurring in Clematis in the USA. American Phytopathological Society Annual Meeting. Phytopathology 104:S3.81.

Interpretive Summary:

Technical Abstract: Clematis is a genus of temperate climbing vines that are popular as ornamentals. Samples from domestic and international sources showing symptoms of yellow mottling and veining, chlorotic ring spots, line pattern mosaics, and in some cases flower distortion and discoloration were received at several diagnostic clinics in the mid-western USA. Spherical virus particles (28-30 nm) were observed by transmission electron microscopy (TEM) in samples associated with symptoms. The virus was detected by TEM and/or RT-PCR in 35 of 80 samples including Clematis spp. 'Barbara', 'Bees Jubilee', ‘Dancing Dorien’, ‘Dancing Smile’, 'Gypsy Queen', 'Henryi', 'Jackmanii', ‘Natascha’, 'Niobe', and ‘Still Waters'. This previously undescribed virus was provisionally named Clematis chlorotic mottle virus (ClCMoV). The ClCMoV ssRNA is approximately 4 kb with four putative open reading frames (ORFs) having similarity to members of Tombusviridae. These ORFs have the highest amino acid sequence similarities (38-59%) to Rosa rugosa leaf distortion virus (RrLDV), Rose yellow leaf virus (RYLV), Pelargonium line pattern virus (PLPV), Pelargonium chlorotic ring pattern virus (PCRPV), Elderberry latent virus (ELV) and Pelargonium ringspot virus (PRSV). Based on these preliminary results ClCMoV appears to be a novel virus in Tombusviridae. Phylogenetic analyses group ClCMoV with RrLDV, RYLV, PLPV, ELV, PRSV, and PCRPV into a distinct group, suggesting a new genus in Tombusviridae may be warranted.