Location: Crop Genetics ResearchTitle: Identification of a soybean rust resistance gene in PI 567104B Author
|Liu, Min - Iowa State University|
|Swaminathan, Sivakumar - Iowa State University|
|Sahu, Binod Bihari - Iowa State University|
|Leandro, Leonor - Iowa State University|
|Cardinal, Andrea - Syngenta|
|Bhattacharyya, Madan Kumar - Iowa State University|
|Cianzio, Silvia - Iowa State University|
Submitted to: Theoretical and Applied Genetics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 12/3/2015
Publication Date: 3/7/2016
Publication URL: http://handle.nal.usda.gov/10113/62114
Citation: Liu, M., Li, S., Swaminathan, S., Sahu, B., Leandro, L., Cardinal, A., Bhattacharyya, M., Song, Q., Walker, D.R., Cianzio, S.R. 2016. Identification of a soybean rust resistance gene in PI 567104B. Theoretical and Applied Genetics. 129:863-877.
Interpretive Summary: Asian soybean rust is one of the most economically important diseases that affect soybean production worldwide. The long-term strategy for minimizing the effects of soybean rust is the development of genetically resistant cultivars to facilitate crop management and protect the environment. In a study to investigate the inheritance of resistance to soybean rust in soybean accession PI 567104B and to fine map the resistance gene using the population with the parentage of IAR 2001 BSR x PI 567104B, we identified a resistance gene to soybean rust. Incorporating the resistance gene into improved soybean cultivars may have wide benefits as it has been shown to have resistance to soybean rust present in the U.S.
Technical Abstract: Asian soybean rust (SBR), caused by the fungus Phakopsora pachyrhizi Syd. & P. Syd., is one of the most economically important diseases that affect soybean production worldwide. A long-term strategy for minimizing the effects of SBR is the development of genetically resistant cultivars integrated with judicious fungicide application to facilitate crop management and protect the environment. The objectives of this study were to map the SBR resistance (Rpp) gene in plant introduction (PI) 567104B and to compare its genomic location with the locations of previously mapped Rpp genes. Using reaction data from field assays of the F2:3 and F4:5 generations from a cross of ‘IAR 2001 BSR’ × PI 567104B, and from a growth chamber assay in which 253 F5:6 recombinant inbred lines (RILs) from the cross were later phenotyped following inoculation with a purified 2006 isolates from Mississippi, a resistance gene locus was mapped to a region containing the Rpp6 locus on chromosome 18. The high level of resistance of F1 seeds from two other crosses in which PI 567104B was used as one of the parents indicated that the gene from PI 567104B was dominant. The interval containing the gene is flanked by the simple sequence repeat (SSR) markers Satt131 and Satt394, and includes the SSR markers BARCSOYSSR_18_0331 and BARCSOYSSR_18_0380. Additional research is needed to investigate the degree of relationship between the allele from PI 567104B and the Rpp6 gene from PI 567102B.