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ARS Home » Midwest Area » Peoria, Illinois » National Center for Agricultural Utilization Research » Mycotoxin Prevention and Applied Microbiology Research » Research » Publications at this Location » Publication #300072
Title: Analysis of fusaric acid in maize using molecularly imprinted solid phase extraction (MISPE) clean-up and ion-pair LC with diode array UV detection

Author
item Appell, Michael
item Jackson, Michael - Mike
item Wang, Lijuan
item HO, CHE-HIN - Central Michigan University
item MUELLER, ANJA - Central Michigan University

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 3/20/2014
Publication Date: 3/20/2014
Citation: Appell, M.D., Jackson, M.A., Wang, L.C., Ho, C., Mueller, A. 2014. Analysis of fusaric acid in maize using molecularly imprinted solid phase extraction (MISPE) clean-up and ion-pair LC with diode array UV detection. Meeting Abstract.

Interpretive Summary:

Technical Abstract: Fusaric acid is a phytotoxin and mycotoxin occasionally found in maize contaminated with Fusarium fungi. A selective sample clean-up procedure was developed to detect fusaric acid in maize using molecularly imprinted solid phase extraction (MISPE) clean-up coupled with ion-pair liquid chromatography and diode array UV detection. Molecularly imprinted polymers (MIPs) were synthesized by thermolytic synthesis with picolinic acid as the mimic template. MP2/6-31+G* ab initio calculations indicated both fusaric acid and picolinic acid interact favorably with the prepolymerization components of the polymers. Imprinted and non-imprinted polymers possessed similar physical properties as characterized by TGA, FT-IR and surface area analysis, and imprinting increased fusaric acid capacity during the loading phase of solid phase extraction. The method developed using an imprinted polymer was suitable for determining fusaric acid levels between 1-100 ug/kg in maize.