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Research Project: Strategies to Control and Prevent Avian Mycoplasmosis

Location: Poultry Research

Title: Stability of rehydrated Mycoplasma gallisepticum vaccine homogeneity over time

Author
item Leigh, Spencer
item Evans, Jeff
item Branton, Scott

Submitted to: International Journal of Poultry Science
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 7/25/2014
Publication Date: 12/20/2014
Citation: Leigh, S.A., Evans, J.D., Branton, S.L. 2014. Stability of rehydrated Mycoplasma gallisepticum vaccine homogeneity over time. International Journal of Poultry Science. 13(10):549-551.

Interpretive Summary: Mycoplasma gallisepticum infection in poultry results in significant loss of revenue for poultry producers. Identifying and following proper vaccination procedures maximizes the efficacy of the vaccine while reducing the cost of vaccination for poultry producers. Spray vaccination is the least expensive method for vaccine application. Part of properly applying the vaccine is maintaining a uniform vaccine solution during vaccination. This work demonstrates that once the vaccine solution is mixed, it will remain homogenous for spraying without further mixing. This information benefits poultry producers as not all vaccination equipment is able to continuously mix the vaccine solution. As the solution does not require continuous mixing, the increased expense of obtaining a vaccinator that provides continuous mixing can be avoided.

Technical Abstract: Proper vaccine application is required to maximize the results of the vaccination, with maintenance of a homogenous solution is critical to obtain uniform results. This study was designed to analyze the need for continued mixing of a Mycoplasma gallisepticum vaccine solution in order to maintain a homogeneous solution during vaccine application. Commercial F-strain vaccine (AviPro® MG F) was rehydrated and diluted in phosphate buffered saline in accordance with field practices. Dextrose was added to the solution to maintain M. gallisepticum viability without growth during the experiment. The vaccine solution was poured into columns, and samples from the static solution were taken from 1, 25, and 50 cm above the base of the column. Samples were taken at 15, 30, 60, and 120 minutes and compared to a control that was mixed prior to sampling. The results indicate that no significant sedimentation occurred over the course of the experiment when comparing the mixed control to any of the samples. These results suggest that there is no need for continuous M. gallisepticum vaccine mixing if vaccine is applied within 2 hours.