Submitted to: Journal of Plant Biology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 1/30/2013
Publication Date: 4/2/2013
Publication URL: http://handle.nal.usda.gov/10113/57990
Citation: Hongmei, Y., Jia, M.H., Jia, Y., Venus, R., Wang, Z., Sun, C., Wang, G. 2013. Molecular mapping of four blast resistance genes using recombinant inbred lines of 93-11 and nipponbare. Journal of Plant Biology. 56:91-97. Interpretive Summary: Mapping of new blast resistance genes is important for developing resistant rice cultivars using marker assisted selection (MAS). Four major quantitative resistant genes for resistance to rice blast disease were mapped with closely linked molecular markers. These new resistance genes can be used to develop new varieties with blast resistance via MAS and to explore a better understanding of molecular basis of rice blast resistance.
Technical Abstract: Molecular mapping of new blast resistance genes is important for developing resistant rice cultivars using marker-assisted selection. In this study, 259 recombinant inbred lines (RILs) were developed from a cross between Nipponbare and 93-11, and were used to construct a 1165.8-cM linkage map with 131 polymorphic simple sequence repeat (SSR) markers. Four major quantitative trait loci (QTLs) for resistance to six isolates of Magnaporthe oryzae were identified: qPi93-1, qPi93-2, qPi93-3, and qPiN-1. For the three genes identified in 93-11, qPi93-1 is linked with SSR marker RM116 on the short arm of chromosome 11 and explains 33% of the phenotypic variation in resistance to isolate CHE86. qPi93-2 is linked with SSR marker RM224 on the long arm of chromosome 11 and accounts for 31% and 25% of the phenotypic variation in resistance to isolates 162-8B and ARB50, respectively. qPi93-3 is linked with SSR marker RM7102 on chromosome 12 and explains 16%, 53%, and 28% of the phenotypic variation in resistance to isolates CHE86, ARB52, and ARB94, respectively. QTL qPiN-1 from Nipponbare is associated with SSR marker RM302 on chromosome 1 and accounts for 34% of the phenotypic variation in resistance to isolate PO6-6. These new genes can be used to develop new varieties with blast resistance via marker-aided selection and to explore the molecular mechanism of rice blast resistance.