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ARS Home » Southeast Area » Athens, Georgia » U.S. National Poultry Research Center » Endemic Poultry Viral Diseases Research » Research » Publications at this Location » Publication #295833

Title: Molecular characterization of turkey enteric coronaviruses circulating in the United States in 2012

item Day, James
item GONDER, ERIC - Butterball, Inc
item JENNINGS, SHANNON - Nash Johnson And Sons
item RIVES, DAVID - Prestage Farms
item TILLEY, BECKY - Butterball, Inc
item WOOMING, BRIAN - Cargill Corporation

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 12/21/2012
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: The use of molecular diagnostic assays has allowed ongoing periodic monitoring of United States turkey flocks for suspected viral enteric pathogens such as reovirus, rotavirus, parvovirus, and astrovirus. Beginning in early 2012, monitoring of commercial turkey flocks in the Southeastern United States via a real-time reverse-transcriptase polymerase chain reaction assay (RT-PCR) revealed a sharp increase in the number of turkey enteric coronavirus (TCoV) positive flocks. Over the course of 2012, numerous observations of suspected turkey coronavirus enteritis were reported from multiple integrators; many of these flocks experiencing enteric disease were positive for TCoV via the RT-PCR assay. This report will focus on the subsequent sequence analysis of the TCoV spike glycoprotein gene and the direct phylogenetic comparisons of TCoV isolates from North Carolina and Arkansas, and will correlate the molecular data with observations from the field and serological data.