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ARS Home » Southeast Area » Mississippi State, Mississippi » Poultry Research » Research » Publications at this Location » Publication #287160
Title: Spray application of live attenuated F Strain-derived Mycoplasma gallisepticum vaccines

Author
item Evans, Jeffrey - Jeff
item JACOB, R - Mississippi State University
item Leigh, Spencer
item Collier, Stephanie
item PEEBLES, E - Mississippi State University
item Branton, Scott

Submitted to: Journal of Applied Poultry Research
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 8/15/2013
Publication Date: 9/15/2013
Citation: Evans, J.D., Jacob, R., Leigh, S.A., Collier, S.D., Peebles, E.D., Branton, S.L. 2013. Spray application of live attenuated F Strain-derived Mycoplasma gallisepticum vaccines. Journal of Applied Poultry Research. 22:842-848.

Interpretive Summary: Live attenuated vaccines (LAVs) are commonly utilized to protect commercial table egg producers from economic losses associated with challenges by the respiratory pathogen Mycoplasma gallisepticum (MG). Currently there are four MG LAVs commercially available within the United States. Consistent among vaccines, protection afforded by the MG LAVs is contingent upon successful vaccine application. Recommended means of application for the four available MG LAVs differ and include drinking water, spray, and eye drop and associated vaccination route may limit an LAV’s suitability for an individual poultry facility. To investigate the impact of altering vaccination routes on vaccine efficacy, two MG LAVs which are considered to be of common origin yet differ in recommended application routes (drinking water vs. spray) were applied to egg layer-type pullets via spray application. Vaccine efficacies were assessed by applying varying doses (0X, 1/100X, 1/10X, 1X, 10X) of each MG LAV and determining the corresponding post-vaccinal immunological responses, LAV-derived in vivo populations, and protection against virulent MG challenge. Research findings demonstrate that when applied by spray application, both MG LAVs resulted in comparable immune responses, viable LAV-derived in vivo populations, and protection against virulent MG challenge. However, for both MG LAVs, dosage rates beyond those recommended by the manufacturers were necessary for consistent effects.

Technical Abstract: Live attenuated vaccines (LAVs) are commonly utilized to protect commercial table egg producers from economic losses associated with challenges by the respiratory pathogen Mycoplasma gallisepticum (MG). Currently there are four MG LAVs commercially available within the United States. Consistent among vaccines, protection afforded by the MG LAVs is contingent upon successful vaccine application. Recommended means of application for the four available MG LAVs differ and include drinking water, spray, and eye drop and associated vaccination route may limit an LAV’s suitability for an individual poultry facility. To investigate the impact of altering vaccination routes on vaccine efficacy, two MG LAVs which are considered to be of common origin yet differ in recommended application routes (drinking water vs. spray) were applied to egg layer-type pullets via spray application. Vaccine efficacies were assessed by applying varying doses (0X, 1/100X, 1/10X, 1X, 10X) of each MG LAV and determining the corresponding post-vaccinal immunological responses, LAV-derived in vivo populations, and protection against virulent MG challenge. Research findings demonstrate that when applied by spray application, both MG LAVs resulted in comparable immune responses, viable LAV-derived in vivo populations, and protection against virulent MG challenge. However, for both MG LAVs, dosage rates beyond those recommended by the manufacturers were necessary for consistent effects.