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ARS Home » Southeast Area » Athens, Georgia » U.S. National Poultry Research Center » Exotic & Emerging Avian Viral Diseases Research » Research » Publications at this Location » Publication #286538

Research Project: Characterization of Protective Host Responses to Avian Influenza Virus Infections in Avian Species

Location: Exotic & Emerging Avian Viral Diseases Research

Title: Adjuvant effects of chitosan and calcium phosphate particles in an inactivated Newcastle disease vaccine

Author
item Volkova, Marina - The All Russian Research Institute For Animal Health (ARRIAH)
item Irza, Anna - The All Russian Research Institute For Animal Health (ARRIAH)
item Chvala, Irina - The All Russian Research Institute For Animal Health (ARRIAH)
item Frolov, Sergy - The All Russian Research Institute For Animal Health (ARRIAH)
item Drygin, Vladimir - The All Russian Research Institute For Animal Health (ARRIAH)
item Kapczynski, Darrell

Submitted to: Avian Diseases
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 9/25/2013
Publication Date: 9/30/2013
Publication URL: http://handle.nal.usda.gov/10113/59841
Citation: Volkova, M., Irza, A., Chvala, I., Frolov, S., Drygin, V., Kapczynski, D.R. 2014. Adjuvant effects of chitosan and calcium phosphate particles in an inactivated Newcastle disease vaccine. Avian Diseases. 58(1):46-52.

Interpretive Summary: Newcastle disease is considered to be one of the most important viral diseases of poultry worldwide. Newcastle disease vaccination is widely practiced in poultry producing countries with the majority of commercial broiler breeders, layers and turkeys receiving multiple vaccinations during their lifetime. The objectives of the present study were to extend the knowledge of protection against virulent Russian Newcastle disease virus by inactivated Newcastle disease virus (NDV) vaccines. The vaccines were formulated with different types of adjuvants to enhance immunity. The results indicate that protection from NDV lethal challenge was dependent on the type of adjuvant added to the inactivated vaccine and thus are important considerations when developing future vaccines.

Technical Abstract: The adjuvant activity of chitosan and calcium phosphate-particles (CAP) was studied following intranasal coadministration of commercial chickens with inactivated Newcastle disease virus (NDV) vaccine. After three vaccinations with inactivated NDV in combination with chitosan or CAP an increase in antibody titers in blood and mucosal samples in chickens was observed when compared with the administration of NDV antigen only. A lower level of humoral immunity was observed in broiler chickens compared to layer-type birds. The chitosan-based vaccine demonstrated higher antigenic and protective activity following lethal challenge than the vaccine containing CAP. Thus, chitosan-particles efficiently changed mucosal and humoral immunity and protective activity makes it possible to use it in the future as a potential adjuvant for production of vaccines for poultry.