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Title: Rapid analysis of lignans from leaves of Podophyllum peltatum l. samples using UPLC-UV-MS

item AVULA, BHARATHI - University Of Mississippi
item WANG, YAN-HONG - University Of Mississippi
item MORAES, RITA - University Of Mississippi
item KHAN, IKHLAS - University Of Mississippi

Submitted to: Biomedical Chromatography
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 11/18/2010
Publication Date: 3/4/2011
Citation: Avula, B., Wang, Y., Moraes, R.M., Khan, I.A. 2011. Rapid analysis of lignans from leaves of Podophyllum peltatum l. samples using UPLC-UV-MS. Biomedical Chromatography. 25:1230-1236.

Interpretive Summary: The new UPLC technique led to a reduction in the mobile phase flow rate; other effects were an increase in acquisition rate and decrease in injection volumes to achieve good peak shapes. The time required for method development, column equilibration or re-equilibration while using gradient elution and for method validation is much shorter compared to conventional HPLC system. This study was conducted to investigate the chemodiversity present at the same location.

Technical Abstract: A new rapid UPLC-UV-MS method has been developed that permits the analysis of four lignans in P. peltatum L. Podophyllotoxin is a natural lignan that is being used as a precursor for the semi-synthetic anti-cancer drugs etoposide, teniposide and etopophos. The chromatographic separation was achieved using a reversed phase C18 column with a mobile phase of water and acetonitrile, both containing formic acid. Analyses of mayapple leaves collected from different colonies within a single site indicated a significant variation in lignan content. The podophyllotoxin content was found in the range of 0.004-0.77 % from the 16 different locations where the samples were collected. LC-mass spectrometry coupled with electrospray ionization (ESI) interface method is described for the identification of four lignans in various populations of plant samples. By applying PCA and HCA, Podophyllum samples collected from various locations were distinguished.