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ARS Home » Midwest Area » St. Paul, Minnesota » Plant Science Research » Research » Publications at this Location » Publication #282886

Title: Investigating nitrogen deficiency in common beans

Author
item O`rourke, Jamie
item Iniguez, Luis - Universidad Nacional Autonoma De Mexico
item Bucciarelli, Bruna
item Woodey, Jenna - Iowa State University
item Shoemaker, Randy
item Graham, Michelle
item Cannon, Steven
item Mcclean, Phillip - North Dakota State University
item Jackson, Scott - University Of Georgia
item Hernandez, Georgina - Universidad Nacional Autonoma De Mexico
item Vance, Carroll

Submitted to: Biennial Conference on Molecular and Cellular Biology of the Soybean
Publication Type: Abstract Only
Publication Acceptance Date: 8/11/2012
Publication Date: 8/13/2012
Citation: O'Rourke, J.A., Iniguez, L.P., Bucciarelli, B., Woodey, J.L., Shoemaker, R.C., Graham, M.A., Cannon, S.B., Mcclean, P.E., Jackson, S.A., Hernandez, G., Vance, C.P. 2012. Investigating nitrogen deficiency in common beans [abstract]. Molecular & Cellular Biology of the Soybean Conference, August 12-15, 2012, Des Moines, Iowa. Available: http://www.extension.iastate.edu/registration/events/soybean/speakeranchor.htm#speaker16.

Interpretive Summary:

Technical Abstract: Phaseolus vulgaris (common bean) and soybean diverged from a common ancestor approximately 19 million years ago. The genome of P. vulgaris is approximately half the size of soybean, making it an excellent model for soybean genetics. Nitrogen (N) is often a growth-limiting nutrient, and N deficiency results in reduced yield. N deficiency is also highly correlated with drought stress, an increasing problem due to global climate change. We performed next generation sequencing (RNAseq) on leaves, roots, and nodules of P. vulgaris cv. Negro jamapa plants that were inoculated with an effective rhizobium, an ineffective rhizobium mutant strain (fix-), or that were provided nitrogen via fertilization. Pathways involved in N assimilation and utilization were examined to determine the effect of fertilization vs. symbiotic N fixation and N deficiency on gene expression patterns. We have identified sequences uniquely expressed in each tissue dependent on the source of available N. Additionally, we have identified the homologous sequences in soybean and have compared gene expression patterns between the two legume species.