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United States Department of Agriculture

Agricultural Research Service


Location: Food and Feed Safety Research

Title: Effects of breed, spermatozoa concentration, and storage on progressive motility of extended boar semen

item Stancic, I
item Dragin, S
item Stancic, B
item Harvey, Roger
item Bozic, A
item Anderson, Robin

Submitted to: Journal of Microbiology, Biotechnology, and Food Science
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 10/10/2011
Publication Date: 12/10/2011
Publication URL:
Citation: Stancic, I., Dragin, S., Stancic, B., Harvey, R.B., Bozic, A., Anderson, R.C. 2011. Effects of breed, spermatozoa concentration, and storage on progressive motility of extended boar semen. Journal of Microbiology, Biotechnology, and Food Science. 1:287-295.

Interpretive Summary: Most swine breeding today is accomplished by artificial insemination, which adds greatly to the efficiency of natural breeding. However, the collection, storage, and use of boar semen could be greatly increased if some procedures were changed. In this study, breed of boar and semen storage practices were evaluated and found to have a great influence on increased fertility in sows. This is important because it can enhance the breeding potential of genetically superior boars and improve profitability for the swine industry.

Technical Abstract: The classic technology of artificial insemination (AI) often requires insemination doses to be kept for more than 24 h, with a requirement that the degree of progressive motility at the moment of insemination not be below 65%. The aim of this paper was to determine the influence of breed, sperm concentration, and storage time on the fertilization capacity of extended semen from native ejaculates of boars. The research included the following boar breeds: Duroc (n=34), Hampshire (n=30), Large White (n=42), and Swedish Landrace (n=32) from large pig farms in Vojvodina (Republic of Serbia). Two ejaculates were collected from each boar once per month for 12 months (a total of 24 ejaculates per boar). There was statistically significant (p<0.01) influence of breed on the number of sperm samples that maintained more than or equal to 65 progressive motility during 48 h of storage in 1:4 dilution. There was also an influence of sperm concentration on progressive motility. As sperm concentration increased during storage, more than or equal to 65% progressive motility declined (P=0.01) within 24 h. The results show that it is necessary to determine the adequate dilution rate and storage time for each ejaculate, while taking into account sperm concentration in the native semen.

Last Modified: 10/20/2017
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