|Avula, Bharathi - University Of Mississippi|
|Wang, Yan-hong - University Of Mississippi|
|Khan, Ikhlas - University Of Mississippi|
Submitted to: Journal of Chromatography
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 12/30/2011
Publication Date: 3/1/2012
Citation: Avula, B., Wang, Y., Khan, I.A. 2012. Quantitative determination of curcuminoids from the Roots of Curcuma longa, Curcuma species and dietary supplements using an UPLC-UV-MS method. Journal of Chromatography. 3(1):1-6.
Interpretive Summary: The method described in this paper is suitable for rapid analysis of curcuminoids and ar-turmerone and for chemical fingerprint analysis. The separation was achieved within 3.5 minutes. The developed method was successfully applied to the identification of five different species of Curcuma (C. zedoaria, C. phaecaulis, C. wenyujin, C. longa and C. kwangsiensis), and dietary supplements claiming to contain C. longa. Liquid chromatography(LC)-mass spectrometry with single quadrupole detector (SQD) is described for the identification of four compounds in plant samples and dietary supplements. Furthermore, the short run time analysis allows increased sample throughput for routine purposes and pharmacokinetic application.
Technical Abstract: A simple, fast UPLC-UV-MS method was developed for the determination of curcuminoids from roots of Curcuma longa L., Curcuma species (C. zedoaria, C. phaecaulis, C. wenyujin and C. kwangsiensis) and dietary supplements claiming to contain C. longa. The total content of curcuminoids (curcumin, desmethoxycurcumin, bisdesmethoxycurcumin) was found to be in the range from 1.16-4.92% and 0.83-35.37% in samples of C. longa and dietary supplements, respectively. LC-mass spectrometry with electrospray ionization (ESI) was used for the identification and confirmation of compounds in various plant samples and dietary supplements.