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ARS Home » Pacific West Area » Davis, California » Crops Pathology and Genetics Research » Research » Publications at this Location » Publication #272927

Title: Detection of Grapevine leafroll-associated virus 7 using real-time PCR and conventional RT-PCR

item AL RWAHNIH, MAHER - University Of California
item OSMAN, FATIMA - University Of California
item Sudarshana, Mysore
item Uyemoto, Jerry
item ROWHANI, ADIB - University Of California

Submitted to: Phytopathology
Publication Type: Abstract Only
Publication Acceptance Date: 6/15/2011
Publication Date: 8/15/2011
Citation: Al Rwahnih, M., Osman, F., Sudarshana, M.R., Uyemoto, J.K., Rowhani, A. 2011. Detection of Grapevine leafroll-associated virus 7 using real-time PCR and conventional RT-PCR. Phytopathology. 101:S4.

Interpretive Summary:

Technical Abstract: Grapevine leafroll-associated virus 7 (GLRaV-7) is an unassigned member in the Closteroviridae family that was first recorded in an asymptomatic white-berried grapevine cultivar from Albania. In California, the virus has been detected in several cultivars including Chardonnay, Merlot, Pinot Noir, Emperor, Black Seedless and Sauvignon Blanc. An ELISA test is available for GLRaV-7, but that assay has been described as unsuitable for field use. In order to improve field detection of this virus, sequences in the coat protein gene (CP) and the heat shock protein homolog gene (hHSP70) from nine Californian GLRaV-7 isolates were compared. Consensus sequences derived from those comparisons were used to design both RT-PCR primers and qRT-PCR assays. When 77 grapevine samples were screened with these two detection methods, the qRT-PCR assay based on the hHSP70 sequence identified more positives (13.86%) than the one based on the CP sequence (9.24%). Both qRT-PCR assays (CP and hHSP70) appeared to be more sensitive than the RT-PCR assay which detected only 2.31%.