Page Banner

United States Department of Agriculture

Agricultural Research Service

Research Project: SUSTAINABLE VINEYARD PRODUCTION SYSTEMS

Location: Crops Pathology and Genetics Research

Title: Analyzing 3D xylem networks in Vitis vinifera using High Resolution Computed Tomography (HRCT))

Author
item Broderson, Craig
item Lee, Eric
item Mcelrone, Andrew
item Shackel, Ken
item Choat, Brendan
item Phillips, Ron
item Matthews, Mark

Submitted to: New Phytologist
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 3/21/2011
Publication Date: 5/13/2011
Publication URL: http://onlinelibrary.wiley.com/doi/10.1111/j.1469-8137.2011.03754.x/pdf
Citation: Broderson, C.R., Lee, E., Mcelrone, A.J., Shackel, K., Choat, B., Phillips, R., Matthews, M.A. 2011. Analyzing 3D xylem networks in Vitis vinifera using High Resolution Computed Tomography (HRCT). New Phytologist. DOI: 10.1111/j.1469-8137.2011.03754.x.

Interpretive Summary: Recent developments in High Resolution Computed Tomography (HRCT) have made it possible to visualize three dimensional (3D) xylem networks without time consuming, labor intensive physical sectioning. Here we describe a new method to visualize complex vessel networks in plants and produce a quantitative analysis of vessel network connectivity. The xylem network of Vitis vinifera L. cv. “Chardonnay” internode sections were visualized using HRCT, revealing a complex intervessel network. Vessel connectivity was verified with SEM imaging and empirical flow measurements. In a 7 mm stem section 80.1% of vessels were connected to at least one other vessel, 50.3% were connected to at least two vessels, and 11.9% were isolated with no connections. Individual vessels, groups of neighboring vessels, or entire stems can be explored with this non-destructive method and allows users to selectively view anatomical features in discrete parts or as a whole. This method will be transformational in our understanding of vessel networks, and how intervessel connectivity influences cavitation and disease resistance.

Technical Abstract: Recent developments in High Resolution Computed Tomography (HRCT) have made it possible to visualize three dimensional (3D) xylem networks without time consuming, labor intensive physical sectioning. Here we describe a new method to visualize complex vessel networks in plants and produce a quantitative analysis of vessel network connectivity. The xylem network of Vitis vinifera L. cv. “Chardonnay” internode sections were visualized using HRCT, revealing a complex intervessel network. Vessel connectivity was verified with SEM imaging and empirical flow measurements. In a 7 mm stem section 80.1% of vessels were connected to at least one other vessel, 50.3% were connected to at least two vessels, and 11.9% were isolated with no connections. Individual vessels, groups of neighboring vessels, or entire stems can be explored with this non-destructive method and allows users to selectively view anatomical features in discrete parts or as a whole. This method will be transformational in our understanding of vessel networks, and how intervessel connectivity influences cavitation and disease resistance.

Last Modified: 8/24/2016
Footer Content Back to Top of Page