Submitted to: Cereal Research Communications
Publication Type: Peer reviewed journal
Publication Acceptance Date: 10/25/2013
Publication Date: 2/21/2014
Citation: Hu, G., Chen, J., Chu, C., Wu, Y. 2014. Mapping of STS markers developed from drought tolerance candidate genes and preliminary analysis of their association with yield-related traits in common wheat (Triticum aestivum). Cereal Research Communications. 42:199-208. Interpretive Summary: Drought stress is a major environmental factor affecting the yield and quality of small grains such as wheat. Understanding the genetic basis of drought tolerance will help plant breeders develop varieties better able to withstand drought. In this study we developed DNA markers for eleven locations on the wheat genome. One of the markers was correlated with grain yield and kernel weight under drought stress conditions. These markers could help wheat breeders develop drought tolerant varieties through a process known as ‘marker assisted selection’.
Technical Abstract: Drought is a severe abiotic stress that affects wheat production worldwide. In order to identify candidate genes for tolerance to water stress in wheat, sequences of 11 genes that have function of drought tolerance in other plant species were used to identify the wheat ortholog genes via homology searching in the wheat EST database. A total of 11 primer pairs were identified and amplified PCR products in wheat. Of them, 10 STS markers were mapped on 11 chromosomes in a set of nulli-tetrasomic lines of ‘Chinese Spring’ wheat; six were mapped on chromosomes 1A, 1B, 4B, 7A, 2B, and 5D, respectively, in a spring wheat mapping population (POP1). The marker XTaABH1 mapped on 7A in POP1 was the only one mapped but characterized in a winter wheat mapping population (POP2) for grain yield, kernel weight and diameter, and height in four field trials applied different water stress or irrigation. The marker XTaABH1 was significantly associated with grain yield under rainfed condition, with kernel weight under terminal and nonirrigation conditions, with kernel diameter and height under non-irrigated condition. The STS primers, map information, and marker-trait association produced in the currently study would be of interest to researchers working on drought tolerance.