Submitted to: National Fusarium Head Blight Forum Proceedings
Publication Type: Proceedings
Publication Acceptance Date: 12/14/2009
Publication Date: 12/7/2009
Citation: Dill-Macky, R., Wennberg, K.J., Scanlan, T.C., Muehlbauser, G.J., Shin, S., Shah, D., Kaur, J., Dahleen, L.S. 2009. Testing Transgenic Spring Wheat and Barley Lines for Reaction to Fusarium Head Blight: 2009 Field Nursery Report. National Fusarium Head Blight Forum Proceedings pg 189 Interpretive Summary:
Technical Abstract: The 2009 field screening nursery, with 128 wheat and 208 barley plots was located at UMore Park, Rosemount MN. Trial entries and untransformed controls were submitted by the University of Minnesota (19+1 wheat), the Donald Danforth Plant Science Center (4+2 wheat) and USDA (48+1 barley). Lines with known reactions to Fusarium head blight (FHB) were also included as checks. The wheat checks used were the moderately resistant Alsen and Tom, the moderately susceptible 2375 and the susceptible cultivars Wheaton and Roblin. The barley checks were the moderately resistant line M122 and the susceptible cultivars Conlon (2-rowed), Robust and Stander. The experimental design was a randomized block with four replicates. Plots were 2.4 m long single rows. The trial was planted on May 6, 2009. All plots, except a non-inoculated Wheaton check, were inoculated twice. The first inoculation was applied at anthesis for wheat and at head emergence for barley. The second inoculation was applied three days after the initial inoculation (dai) for each plot. The inoculum was a composite of 50 F. graminearum isolates at a concentration of 200,000 macroconidia.ml-1 with Tween 20 (polysorbate) added at 2.5 ml.L-1 as a wetting agent. The inoculum was applied using a CO2-powered backpack sprayer fitted with a SS8003 TeeJet spray nozzle with an output of 10ml.sec-1 at a working pressure of 275 kPa. Mist-irrigation was applied from the first inoculation on June 26 till July 26 to facilitate FHB development. FHB incidence and severity were assessed visually 20-21 d.a.i. for wheat and 13-14 d.a.i. for barley on 20 arbitrarily selected spikes per plot. FHB incidence was determined by the percentage of spikes with visually symptomatic spikelets of the 20 spikes observed. FHB severity was determined as the percentage symptomatic spikelets of the total of all spikelets observed in these 20 spikes. Plots were harvested at maturity on August 14 (barley) and 24 (wheat). The harvested seed from each plot was split to obtain a 25 g sub-sample, which was then cleaned by hand. The wheat sub-samples were used to estimate the percentage of visually scabby kernels (VSK) and then all samples (wheat and barley) were ground and submitted for deoxynivalenol (DON) analysis. The data indicated that resistance was expressed in some of the transformed lines.