|ROY-CHOWDHURY, MOYTRI - University Of Arkansas|
|CARTWRIGHT, RICK - University Of Arkansas|
Submitted to: American Phytopathological Society Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 2/7/2020
Publication Date: 2/7/2010
Citation: Roy-Chowdhury, M., Jia, Y., Jackson, A.K., Jia, M.H., Fjellstrom, R.G., Cartwright, R. 2010. Characterization of rice blast resistance gene Pi-z (t) in rice germplasm using DNA markers and Pathogenicity assays. In: 87th Meeting of the Southern Division of the American Phytopathological Society, Orlando, FL, February 7-8-2010.
Technical Abstract: The Pi-z(t) gene in rice confers resistance to a wide range of races of the rice blast fungus, Magnaporthe oryzae. The objective of the present study was to identify Pi-z(t) in 131 worldwide rice germplasm accessions using DNA markers and pathogenicity assays. Four simple sequence repeat (SSR) markers (RM527, AP4791, AP5659-1, AP5659-5) closely linked to Pi-z(t) were first used to predict the existence of Pi-z(t) in rice germplasm and results were verified using pathogenicity assays with an avirulent strain (IE1k) and two virulent races, IB33 and IB49. The presence of the Pi-z(t) gene in ninety-eight germplasm accessions out of 131 was verified using both SSR markers and pathogenicity assays. The remaining 33 germplasm accessions containing different SSR alleles were found to be different in their responses to blast races, IB33, IEIk and IB49 suggesting the presence of additional R gene(s) in these germplasm. These characterized germplasm should be useful for genetic studies and marker assisted breeding for improving blast resistance worldwide.