Location: Cereal Crops ResearchTitle: Quantitative Trait Loci Influencing Grain Hardness in Hard Red Spring Wheat Breeding Lines MN98550 and MN99394) Author
Submitted to: Meeting Abstract
Publication Type: Abstract only
Publication Acceptance Date: 8/3/2009
Publication Date: 11/1/2009
Citation: Tsilo, T.J., Hareland, G.A., Chao, S., Simsek, S., Anderson, J.A. 2009. Quantitative Trait Loci Influencing Grain Hardness in Hard Red Spring Wheat Breeding Lines MN98550 and MN99394. Meeting Abstract 167-7 Interpretive Summary:
Technical Abstract: Quantitative trait loci (QTL) affecting endosperm texture were mapped in hard red spring wheat breeding lines adapted to the Upper Midwest region of the United States. Mapping was conducted using a population of 139 recombinant inbred lines (RILs) developed from the MN98550 x MN99394 cross. Lines grown at three Minnesota locations in 2006 were tested for endosperm texture using the single kernel characteristics system (SKCS). RILs were genotyped with 279 SSR markers. The previously known hardness (Ha) locus on chromosome 5DS explained 17, 21, and 20% of the total phenotypic variation in endosperm texture in all the three environments, Crookston, Morris, and St. Paul, respectively. The puroindoline specific markers at the hardness locus were previously reported by several groups and are already in use in breeding programs. In this study, a second QTL, detected on chromosome 5A, explained 11, 10, and 12% of the total phenotypic variation in endosperm texture in all the three environments, Crookston, Morris, and St. Paul, respectively. The 5A QTL also coincided with a grain protein QTL. Availability of molecular markers linked to QTL for endosperm texture will be useful in increasing the frequency of desirable alleles and also for selecting increased hardness among hard wheats in early generations of breeding populations.