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ARS Home » Pacific West Area » Davis, California » Crops Pathology and Genetics Research » Research » Publications at this Location » Publication #238474

Title: LETTUCE INFECTIOUS YELLOWS VIRUS-ENCODED P26 INDUCES PLASMALEMMA DEPOSIT CYTOPATHOLOGY

Author
item Stewart, Lucy - University Of California
item Medina, Vicente - Universitat De Lleida
item Sudarshana, Mysore
item Falk, Bryce - University Of California

Submitted to: Virology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 3/17/2009
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Lettuce infectious yellows virus (LIYV) encodes a 26 kDa protein (P26) previously shown to associate with plasmalemma deposits (PLDs), unique LIYV-induced cytopathologies located at the plasmalemma over plasmodesmata pit fields in companion cells and phloem parenchyma. To further characterize the relationship of P26 and PLDs, we assessed localization and cytopathology induction of P26 expressed from either LIYV or a heterologous Tobacco mosaic virus (TMV) vector using green fluorescent protein (GFP) fusions, immunofluorescence microscopy, biochemical fractionation, and transmission electron microscopy (TEM). TEM analyses demonstrated that P26 not only associated with, but induced formation of PLDs in the absence of other LIYV proteins. Interestingly, PLDs induced by P26-expressing TMV were no longer confined to phloem cells. Putative P26 orthologs from two other members of the genus Crinivirus which do not induce conspicuous PLDs exhibited fractionation properties similar to LIYV P26 but were not associated with any PLD-like cytopathology.