Submitted to: Genbank
Publication Type: Other
Publication Acceptance Date: 6/4/2008
Publication Date: 6/4/2008
Citation: Yocum, G.D., Rinehart, J.P., Chirumamilla, A., Larson, M.L. 2008. Isolation of diapause-regulated transcripts by differential display from the Colorado potato beetle, and their expression in prediapausing and nondiapausing adults. GenBank. Accessions: FG591137-FG591192.
Technical Abstract: Using differential display, 56 putatively diapause regulated transcripts were isolated from the Colorado potato beetle, Leptinotarsa decemlineata. The clones insert sizes range from 114 to 795 bp with mean length of 392 ± SD of 191 bp. Fourteen of the transcripts were confirmed by northern blot analysis to be diapause regulated. Blast searches assigned the diapause-regulated transcripts putative identities falling into five broad categories of proteins: glycine-rich, digestive, defensive, structural, and those of unknown function. The transcripts were highly expressed for the first 13 to 15 days postemergence in prediapausing adults and were then significantly down-regulated in diapausing beetles. The diapause-down-regulated transcripts were also highly expressed for the first seven days postemergence in nondiapausing adults and were down-regulated on day 9 or 11 in most individuals examined. A comparison of the transcript expression patterns in prediapausing and nondiapausing adults showed that the expression of the transcripts persisted for approximately four days longer in the prediapausing beetles.