Submitted to: Natural Product Communications
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 5/23/2008
Publication Date: 5/7/2008
Citation: Tabanca, N., Wedge, D.E., Wang, X., Demirci, B., Baser, K., Zhou, L., Cutler, S.J. 2008. Chemical Composition and Antifungal Activity of Angelica sinensis Essential Oil Against Three Colletotrichum Species. Natural Product Communications. 3(7):1073-1078. Interpretive Summary: Development of natural product-based agrochemicals from plants used in traditional medicine systems offers an opportunity to find new chemistry with reduced environmental and health hazards for agriculture. As part of our program to discover novel natural product based fungicides for agriculture, several sensitive assay systems were developed to evaluate naturally occurring antifungal agents in plant, fungal, and marine extracts. In this study, we investigated the chemical composition of A. sinensis root essential oil by GC and GC-MS techniques and identified antifungal properties of Angelica oil using bioautography and detached leaf bioassays.
Technical Abstract: Chemical fungicides are an important component in disease management for most crops. As part of a program to discover natural product-based fungicides, several sensitive assay systems have been developed for the evaluation of naturally occurring antifungal agents. In this study, we focused on the discovery and evaluation of new natural product-based fungicides from Angelica sinensis (Dong Quai) for use in control of strawberry anthracnose caused by Colletotrichum species. Angelica sinensis oil was evaluated for antifungal activity against three Colletotrichum species using direct-bioautography assay and a strawberry detached leaf assay using Colletotrichum fragariae. Chemical composition and fingerprinting were conducted by gas chromatography and gas chromatography-mass spectrometry. Major components were identified as (Z)-ligustilide (43.1%), (E)-3-butylidene phthalide (14.5%), (Z)B-ocimene (12.9%), and apiole (11.2%).