Submitted to: Plant and Animal Genome Conference
Publication Type: Abstract Only
Publication Acceptance Date: 10/15/2007
Publication Date: 1/11/2008
Citation: Yu, G., Harris, M.O., Cai, X., Xu, S.S. 2008. Saturation Mapping of Hessian Fly Resistance Gene H26 in Synthetic Wheat. Plant and Animal Genome Conference. Jan 12-16, 2008 San Diego Ca pg 309
Technical Abstract: Hessian fly (Mayetiola destructor) resistance gene H26, derived from Aegilops tauschii, is one of the most effective R genes against various biotypes of Hessian fly. Using a limited number of PCR-based molecular markers, a previous study mapped H26 to the chromosomal deletion bin 3DL3-0.81-1.00. The objective of this study was to saturate this chromosomal region harboring H26 with newly-developed PCR-based markers. A population of 96 F2 individuals was used for saturation mapping in this study. This population was developed from the cross between resistant synthetic hexaploid wheat lines SW8 (Langdon/Ae. tauschii CIae 25) and the susceptible line SW11 (Langdon/Ae. tauschii H-80-114-1). All ESTs assigned in the physical bin 3DL3-0.81-1.00 were used to design PCR-based STS primers using program Primer 3. To date, more than 20 newly-developed STS markers have been mapped to chromosomal region spanning the H26 locus. One of the STS markers has a distance of 1 cM from the H26 locus. The newly developed STS markers closely linked to H26 will be useful for marker-assisted selection in wheat breeding as well as high resolution mapping for further study of the H26 gene. They will also facilitate the genetic study of chromosome 3D, which currently has fewer PCR-based DNA markers than chromosomes 3A and 3B.