Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 10/2/2006
Publication Date: 1/13/2007
Citation: Li, J., Klindworth, D.L., Cai, X., Hu, J., Xu, S.S. 2007. Molecular characterization of Langdon durum D-genome disomic substitution lines. Plant and Animal Genome VX Conference Abstracts. P 200. p. 151.
Technical Abstract: The aneuploid stocks of durum wheat and common wheat have been developed mainly in 'Langdon' (LDN) and 'Chinese Spring' (CS), respectively. The LDN-CS D-genome chromosome disomic substitution (LDN-DS) lines, where a pair of CS D-genome chromosomes substitute for a corresponding homoeologous A- or B-genome chromosome pair of LDN, have been widely used for determining chromosomal locations of genes in tetraploid wheat. The objectives of this study were to characterize a set of 14 LDN-DS lines and develop chromosome-specific markers using TRAP (target region amplification polymorphism) marker technique. In this study, a total of 307 polymorphic DNA fragments were amplified from LDN and CS and 302 of them were assigned to individual chromosomes. Most of the markers (95.5%) were present on a single chromosome as chromosome-specific markers, but 4.5% of the markers mapped to two or more chromosomes. The number of markers per chromosome varied from 10 (chromosomes 1A and 6D) through 24 (chromosome 3A). The TRAP marker analysis verified the retention of at least 13 pairs of A- or B-genome chromosomes from LDN and one pair of D-genome chromosomes from CS in each of the LDN-DS lines. The chromosome-specific markers developed in this study provide an identity for individual wheat chromosomes and will facilitate genetic and genomic analysis in wheat.